Rabbit anti-Human, Mouse RNF6 Polyclonal Antibody | anti-RNF6 antibody

RNF6 Rabbit Polyclonal

Cat. Num. AAA7603593

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: RNF6; Polyclonal Antibody; RNF6 Rabbit Polyclonal; anti-RNF6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 142

• Applicable Applications for anti-RNF6 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Immunogen: Ring finger protein (C3H2C3 type) 6
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Western Blot (WB)

(mouse testis tissue were subjected to SDS PAGE followed by western blot with MBS7603593 (RNF6 antibody) at dilution of 1:800)

NCBI and Uniprot Product Information

• NCBI GI #: 12656363
• NCBI GeneID: 6049
• Molecular Weight: 16,680 Da
• NCBI Official Full Name: RNF6 protein
• NCBI Official Synonym Full Names: ring finger protein 6
• NCBI Official Symbol: RNF6
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF6
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF6
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF6
• UniProt Synonym Gene Names: SPG2
• UniProt Entry Name: RNF6_HUMAN

NCBI Description

The protein encoded by this gene contains a RING-H2 finger motif. Deletions and mutations in this gene were detected in esophageal squamous cell carcinoma (ESCC), suggesting that this protein may be a potential tumor suppressor. Studies of the mouse counterpart suggested a role of this protein in the transcription regulation that controls germinal differentiation. Multiple alternatively spliced transcript variants encoding the same protein are observed. [provided by RefSeq, Jul 2008]

Uniprot Description

RNF6: E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation. Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover. Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator. Defects in RNF6 may be a cause of esophageal cancer (ESCR). A malignancy of the esophagus. The most common types are esophageal squamous cell carcinoma and adenocarcinoma. Cancer of the esophagus remains a devastating disease because it is usually not detected until it has progressed to an advanced incurable stage. Belongs to the RNF12 family.

Protein type: Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 13q12.2

Cellular Component: axon; cytoplasm; intracellular membrane-bound organelle; nuclear membrane; nucleoplasm; nucleus; PML body

Molecular Function: androgen receptor binding; DNA binding; ligase activity; protein binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: negative regulation of axon extension; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; regulation of transcription, DNA-dependent; ubiquitin-dependent protein catabolic process

Disease: Esophageal Cancer