Testing Data
(Gel: 10%SDS-PAGE Lysates (from left to right): Jurkat, 231 and K562 cell Amount of lysate: 40ug per lane Primary antibody: 1/200 dilution Secondary antibody dilution: 1/8000 Exposure time: 3 minutes)
Rabbit RNF5 Polyclonal Antibody | anti-RNF5 antibody
RNF5 Antibody
Immunohistochemistry: 1:25-1:100
Testing Data
(Gel: 10%SDS-PAGE Lysates (from left to right): Jurkat, 231 and K562 cell Amount of lysate: 40ug per lane Primary antibody: 1/200 dilution Secondary antibody dilution: 1/8000 Exposure time: 3 minutes)
Testing Data
(Gel: 10%SDS-PAGE Lysates (from left to right): Jurkat, 231 and K562 cell Amount of lysate: 40ug per lane Primary antibody: 1/200 dilution Secondary antibody dilution: 1/8000 Exposure time: 3 minutes)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/35.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/35.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions. [provided by RefSeq, Jul 2008]
Uniprot Description
RNF5: Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses.
Protein type: Ubiquitin conjugating system; Ligase; Membrane protein, multi-pass; Ubiquitin ligase; Membrane protein, integral; EC 6.3.2.19; EC 6.3.2.-
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: endoplasmic reticulum membrane; mitochondrial membrane; integral to membrane
Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity
Biological Process: negative regulation of autophagy; protein destabilization; response to bacterium; cellular protein catabolic process
Research Articles on RNF5
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Product Notes
The RNF5 rnf5 (Catalog #AAA9409335) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNF5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RNF5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500-1:2000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the RNF5 rnf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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