Rabbit RNF43 Polyclonal Antibody | anti-RNF43 antibody

Anti-RNF43 Picoband Antibody

Cat. Num. AAA1750696

• Gene Names: RNF43; URCC; SSPCS; RNF124
• Reactivity: human, Mouse, Rat; No cross reactivity with other proteins.
• Applications: ELISA, Western Blot
• Purity: Immunogen affinity purified
• Synonyms: RNF43; Polyclonal Antibody; Anti-RNF43 Picoband Antibody; E3 ubiquitin-protein ligase RNF43; RING finger protein 43; RING-type E3 ubiquitin transferase RNF43; anti-RNF43 antibody

• Host: Rabbit
• Reactivity: human, Mouse, Rat; No cross reactivity with other proteins.
• Clonality: Polyclonal
• Purity/Purification: Immunogen affinity purified
• Form/Format: Lyophilized
• Sequence Length: 869

• Applicable Applications for anti-RNF43 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 0.1-0.5mug/ml; Direct ELISA: 0.1-0.5mug/ml; ; Tested Species: In-house tested species with positive results.; ; Other applications have not been tested.; Optimal dilutions should be determined by end users.
• Immunogen: E Coli-derived human RNF43 recombinant protein (Position: G24-Y197).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Preparation and Storage: Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Related Product Information for anti-RNF43 antibody

Description: RNF43 is a HAP95 (AKAP8L) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer. The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms.
Protein Function: E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.

NCBI and Uniprot Product Information

• NCBI GI #: 770075665
• NCBI GeneID: 54894
• NCBI Accession #: NP_001292473.1
• NCBI GenBank Nucleotide #: NM_001305544.1
• UniProt Accession #: Q68DV7; Q65ZA4; Q6AI04; Q9NXD0; A8K4R2; B7Z443; B7Z5D5; B7Z5J5
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF43 isoform 1
• NCBI Official Synonym Full Names: ring finger protein 43
• NCBI Official Symbol: RNF43
• NCBI Official Synonym Symbols: URCC; SSPCS; RNF124
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF43
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF43
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF43

NCBI Description

The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]

Uniprot Description

E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.