Rabbit anti-Human GRIK2 Polyclonal Antibody | anti-GRIK2 antibody

GRIK2, CT (GRIK2, GLUR6, Glutamate receptor, ionotropic kainate 2, Excitatory amino acid receptor 4, Glutamate receptor 6) (APC)

Cat. Num. AAA6304383

• Gene Names: GRIK2; EAA4; GLR6; MRT6; GLUK6; GLUR6; GluK2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GRIK2; Polyclonal Antibody; CT (GRIK2; GLUR6; Glutamate receptor; ionotropic kainate 2; Excitatory amino acid receptor 4; Glutamate receptor 6) (APC); Glutamate receptor 6; anti-GRIK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-GRIK2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GRIK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 827-856 amino acids from the C-terminal region of human GRIK2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GRIK2 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GRIK2 antibody detected the GRIK2 protein (arrow).)

Related Product Information for anti-GRIK2 antibody

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing at multiple sites within the first and second transmembrane domains, which is thought to alter the structure and function of the receptor complex. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. Mutations in this gene have been associated with autosomal recessive mental retardation.

Product Categories/Family for anti-GRIK2 antibody

Antibodies; Receptors

References

Han, Y., et al. Biochemistry 49(43):9207-9216(2010). Holt, R., et al. Eur. J. Hum. Genet. 18(9):1013-1019(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Sampaio, A.S., et al. CNS Neurosci Ther (2010). In press: Sander, T., et al. Neurology 45(9):1713-1720(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 28559003
• NCBI GeneID: 2898
• NCBI Accession #: NP_786944.1; NP_068775.1; NP_001159719.1
• NCBI GenBank Nucleotide #: NM_175768.3
• UniProt Accession #: Q13002; Q8WWS1; Q96KS6; Q96KS7; Q96KS8; A6NMY9; B5MCV0; D7RWZ3; D7RWZ4; D7RWZ5; D7RWZ6; D7RWZ7
• Molecular Weight: 77,112 Da
• NCBI Official Full Name: glutamate receptor ionotropic, kainate 2 isoform 2
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, kainate 2
• NCBI Official Symbol: GRIK2
• NCBI Official Synonym Symbols: EAA4; GLR6; MRT6; GLUK6; GLUR6; GluK2
• NCBI Protein Information: glutamate receptor ionotropic, kainate 2; gluR-6; bA487F5.1; glutamate receptor 6; glutamate receptor form A; glutamate receptor form B; glutamate receptor form C; glutamate receptor form D; glutamate receptor form E; excitatory amino acid receptor 4
• UniProt Protein Name: Glutamate receptor ionotropic, kainate 2
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: GRIK2
• UniProt Synonym Gene Names: GLUR6; GluK2; EAA4; GluR-6; GluR6
• UniProt Entry Name: GRIK2_HUMAN

NCBI Description

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing at multiple sites within the first and second transmembrane domains, which is thought to alter the structure and function of the receptor complex. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. Mutations in this gene have been associated with autosomal recessive mental retardation. [provided by RefSeq, Jul 2008]

Uniprot Description

GluR6: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Modulates cell surface expression of NETO2. Defects in GRIK2 are the cause of mental retardation autosomal recessive type 6 (MRT6). It is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic mental retardation. MRT6 patients display mild to severe mental retardation and psychomotor development delay in early childhood. Patients do not have neurologic problems, congenital malformations, or facial dysmorphism. Body height, weight, and head circumference are normal. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK2 subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Channel, calcium; Channel, ligand-gated; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 6q16.3

Cellular Component: dendrite cytoplasm; presynaptic membrane; postsynaptic membrane; kainate selective glutamate receptor complex; integral to plasma membrane; dendrite; postsynaptic density; plasma membrane; terminal button; perikaryon; cell junction

Molecular Function: protein homodimerization activity; kainate selective glutamate receptor activity; extracellular-glutamate-gated ion channel activity; ubiquitin conjugating enzyme binding; ubiquitin protein ligase binding; PDZ domain binding

Biological Process: regulation of synaptic transmission; regulation of long-term neuronal synaptic plasticity; synaptic transmission, glutamatergic; behavioral fear response; glutamate signaling pathway; generation of action potential; negative regulation of synaptic transmission, glutamatergic; regulation of short-term neuronal synaptic plasticity; regulation of JNK cascade; receptor clustering; positive regulation of synaptic transmission; cellular calcium ion homeostasis; synaptic transmission; intracellular protein transport; regulation of inhibitory postsynaptic membrane potential; neuron apoptosis; ionotropic glutamate receptor signaling pathway; transport; positive regulation of neuron apoptosis; negative regulation of neuron apoptosis; regulation of excitatory postsynaptic membrane potential

Disease: Mental Retardation, Autosomal Recessive 6