Rabbit anti-Human RNF19A Polyclonal Antibody | anti-RNF19A antibody

RNF19A, ID (RNF19A, RNF19, E3 ubiquitin-protein ligase RNF19A, Double ring-finger protein, RING finger protein 19A, p38) (Biotin)

Cat. Num. AAA6342766

• Gene Names: RNF19A; RNF19
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RNF19A; Polyclonal Antibody; ID (RNF19A; RNF19; E3 ubiquitin-protein ligase RNF19A; Double ring-finger protein; RING finger protein 19A; p38) (Biotin); p38; anti-RNF19A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RNF19A antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RNF19A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383-413 amino acids from the Central region of human RNF19A.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RNF19A Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RNF19A antibody detected the RNF19A protein (arrow).)

Immunohistochemistry (IHC)

(RNF19A Antibody (Center) (AP14232c)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RNF19A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-RNF19A antibody

The protein encoded this gene contains two RING-finger motifs and an IBR (in between RING fingers) motif. This protein is an E3 ubiquintin ligase that is localized in Lewy bodies (LBs), a characteristic neuronal inclusion in Parkinson's disease (PD) brains. This protein interacts with UBE2L3/UBCH7 and UBE2E2/UBCH8, but not other ubiquitin-conjugating enzymes. This protein is found to bind and ubiquitylate synphilin 1 (SNCAIP), which is a interacting protein of alpha synuclein in neurons, and a major component of LB. Alternatively spliced transcript variants encoding the same protein have been reported.

Product Categories/Family for anti-RNF19A antibody

Antibodies; Ubiquitin Ligases

References

Huh, J.W., et al. Gene 424 (1-2), 63-70 (2008): Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Ishigaki, S., et al. Neurobiol. Dis. 25(2):331-341(2007). Huang, Y., et al. J. Biol. Chem. 281(17):11610-11617(2006). Ishigaki, S., et al. J. Biol. Chem. 279(49):51376-51385(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 35493782
• NCBI GeneID: 25897
• NCBI Accession #: NP_056250.3; NP_904355.1
• NCBI GenBank Nucleotide #: NM_015435.4
• UniProt Accession #: Q9NV58; Q52LG1; Q9H5H9; Q9H8M8; Q9UFG0; Q9UFX6; Q9Y4Y1; A3KCU9
• Molecular Weight: 87,256 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF19A
• NCBI Official Synonym Full Names: ring finger protein 19A, RBR E3 ubiquitin protein ligase
• NCBI Official Symbol: RNF19A
• NCBI Official Synonym Symbols: RNF19
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF19A
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF19A
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF19A
• UniProt Synonym Gene Names: RNF19; Dorfin
• UniProt Entry Name: RN19A_HUMAN

NCBI Description

This gene encodes a member of the ring between ring fingers (RBR) protein family, and the encoded protein contains two RING-finger motifs and an in between RING fingers motif. This protein is an E3 ubiquitin ligase that is localized to Lewy bodies, and ubiquitylates synphilin-1, which is an interacting protein of alpha synuclein in neurons. The encoded protein may be involved in amyotrophic lateral sclerosis and Parkinson's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]