Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using RNF180 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)
Rabbit anti-Human RNF180 Polyclonal Antibody | anti-RNF180 antibody
RNF180 Polyclonal Antibody
Gene Names
RNF180; RINES
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purification
Synonyms
RNF180; Polyclonal Antibody; RNF180 Polyclonal Antibody; RINES; anti-RNF180 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
MKRSKELITKNHSQEETSILRCWKCRKCIASSGCFMEYLENQVIKDKDDSVDAQNICHVWHMNVEALPEWISCLIQKAQWTVGKLNCPFCGARLGGFNFVSTPKCSCGQLAAVHLSKSRTDYQPTQAGRLMRPSVKYLSHPRVQSGCDKEALLTGGGSENRNHRLLNMARNNNDPGRLTEALCLEVRPTYFEMKNEKLLSKASEPKYQLFVPQLVTGRCATRAFHRKSHSLDLNISEKLTLLPTLYEIHSKTTAY
Sequence Length
592
Applicable Applications for anti-RNF180 antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Immunogen
Recombinant protein of human RNF180
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Endoplasmic reticulum membrane, Nucleus envelope, Single-pass membrane protein
Positive Samples
Jurkat, mouse brain, mouse testis, rat brain
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using RNF180 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using RNF180 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)
Product Categories/Family for anti-RNF180 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 11kDa; 47kDa; 68kDa
Observed: 65kDa
Observed: 65kDa
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF180 isoform 1
NCBI Official Synonym Full Names
ring finger protein 180
NCBI Official Symbol
RNF180
NCBI Official Synonym Symbols
RINES
NCBI Protein Information
E3 ubiquitin-protein ligase RNF180
UniProt Protein Name
E3 ubiquitin-protein ligase RNF180
Protein Family
UniProt Gene Name
RNF180
Uniprot Description
E3 ubiquitin-protein ligase which promotes polyubiquitination and degradation by the proteasome pathway of ZIC2.
Research Articles on RNF180
Similar Products
Product Notes
The RNF180 rnf180 (Catalog #AAA9135227) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNF180 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNF180 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the RNF180 rnf180 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MKRSKELITK NHSQEETSIL RCWKCRKCIA SSGCFMEYLE NQVIKDKDDS VDAQNICHVW HMNVEALPEW ISCLIQKAQW TVGKLNCPFC GARLGGFNFV STPKCSCGQL AAVHLSKSRT DYQPTQAGRL MRPSVKYLSH PRVQSGCDKE ALLTGGGSEN RNHRLLNMAR NNNDPGRLTE ALCLEVRPTY FEMKNEKLLS KASEPKYQLF VPQLVTGRCA TRAFHRKSHS LDLNISEKLT LLPTLYEIHS KTTAY. It is sometimes possible for the material contained within the vial of "RNF180, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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