Rabbit RNF169 Polyclonal Antibody | anti-RNF169 antibody
Anti-RNF169 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Immunogen Affinity Purified
Synonyms
RNF169; Polyclonal Antibody; Anti-RNF169 Antibody; E3 ubiquitin-protein ligase RNF169; ring finger protein 169; RING finger protein 169; RING-type E3 ubiquitin transferase RNF169; KIAA1991; anti-RNF169 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized
Sequence Length
708
Applicable Applications for anti-RNF169 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)
Application Notes
WB: 0.1-0.5ug/ml
IHC-P: 0.5-1ug/ml
Direct ELISA: 0.1-0.5ug/ml
IHC-P: 0.5-1ug/ml
Direct ELISA: 0.1-0.5ug/ml
Immunogen
E Coli-derived human RNF169 recombinant protein (Position: N311-K708).
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Relevant Detection Systems
Relevant Detection Systems
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500mug/ml.
Relevant Detection Systems
Relevant Detection Systems
Relevant Detection Systems
We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit for IHC(P).
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Related Product Information for anti-RNF169 antibody
Description: Rabbit IgG polyclonal antibody for RNF169 detection. Tested with WB, IHC-P, Direct ELISA in Human; Mouse; Rat.
Background: RING finger protein 169 (RNF169) is a probable E3 ubiquitin-protein ligase that acts as a negative regulator of the repair of double-strand breaks (DSBs) following DNA damage. RNF169 is recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSB repair. This gene is mapped to 11q13.4.
Background: RING finger protein 169 (RNF169) is a probable E3 ubiquitin-protein ligase that acts as a negative regulator of the repair of double-strand breaks (DSBs) following DNA damage. RNF169 is recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSB repair. This gene is mapped to 11q13.4.
References
1. Chen J, Feng W, Jiang J, Deng Y, Huen MS. Ring finger protein RNF169 antagonizes the ubiquitin-dependent signaling cascade at sites of DNA damage. J Biol Chem. 2012 Aug 10;287(33):27715-22. doi: 10.1074/jbc.M112.373530. Epub 2012 Jun 25.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF169
NCBI Official Synonym Full Names
ring finger protein 169
NCBI Official Symbol
RNF169
NCBI Protein Information
E3 ubiquitin-protein ligase RNF169
UniProt Protein Name
E3 ubiquitin-protein ligase RNF169
Protein Family
UniProt Gene Name
RNF169
UniProt Synonym Gene Names
KIAA1991
UniProt Entry Name
RN169_HUMAN
Research Articles on RNF169
1. results highlight the interplay of RNF169 with 53BP1 in fine-tuning choice of DSB repair pathways.
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Product Notes
The RNF169 rnf169 (Catalog #AAA1752551) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RNF169 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RNF169 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA). WB: 0.1-0.5ug/ml IHC-P: 0.5-1ug/ml Direct ELISA: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the RNF169 rnf169 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF169, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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