Western Blot (WB)
(RNF160 Antibody (Center) (MBS9215022) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RNF160 antibody detected the RNF160 protein (arrow).)
Rabbit anti-Human RNF160 Polyclonal Antibody | anti-LTN1 antibody
RNF160 Antibody (Center)
Gene Names
LTN1; RNF160; ZNF294; C21orf10; C21orf98
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RNF160; Polyclonal Antibody; RNF160 Antibody (Center); C21orf10; C21orf98; KIAA0714; ZN; anti-LTN1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RNF160 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1087-1115 amino acids from the Central region of human RNF160.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
1087-1115
Sequence Length
1812
Applicable Applications for anti-LTN1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Function
E3 ubiquitin-protein ligase component of theribosome quality control complex (RQC), aribosome-associated complex that mediatesubiquitination and extraction of incompletelysynthesized nascent chains for proteasomaldegradation (PubMed: 23685075, PubMed: 25132172, PubMed: 25578875).Ubiquitination leads to VCP/p97 recruitment for extraction and degradation of the incomplete translation product (By similarity).
Preparation and Storage
Maintain refrigerated at 2-8°C. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(RNF160 Antibody (Center) (MBS9215022) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RNF160 antibody detected the RNF160 protein (arrow).)
Western Blot (WB)
(RNF160 Antibody (Center) (MBS9215022) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RNF160 antibody detected the RNF160 protein (arrow).)
Related Product Information for anti-LTN1 antibody
Like most RING finger proteins, LTN1 functions as an E3
ubiquitin ligase (Chu et al., 2009 [PubMed 19196968]).[supplied by
OMIM].
ubiquitin ligase (Chu et al., 2009 [PubMed 19196968]).[supplied by
OMIM].
Product Categories/Family for anti-LTN1 antibody
References
Bengtson, M.H., et al. Nature 467(7314):470-473(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Chu, J., et al. Proc. Natl. Acad. Sci. U.S.A. 106(7):2097-2103(2009)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Chu, J., et al. Proc. Natl. Acad. Sci. U.S.A. 106(7):2097-2103(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
200552
NCBI Official Full Name
E3 ubiquitin-protein ligase listerin
NCBI Official Synonym Full Names
listerin E3 ubiquitin protein ligase 1
NCBI Official Symbol
LTN1
NCBI Official Synonym Symbols
RNF160; ZNF294; C21orf10; C21orf98
NCBI Protein Information
E3 ubiquitin-protein ligase listerin
UniProt Protein Name
E3 ubiquitin-protein ligase listerin
UniProt Gene Name
LTN1
UniProt Synonym Gene Names
C21orf10; C21orf98; KIAA0714; RNF160; ZNF294
UniProt Entry Name
LTN1_HUMAN
NCBI Description
Like most RING finger proteins, LTN1 functions as an E3 ubiquitin ligase (Chu et al., 2009 [PubMed 19196968]).[supplied by OMIM, Nov 2010]
Uniprot Description
ZNF294: a zinc-finger protein which contains one RING/zf-C3HC4 domain. The RING finger is a cysteine-rich domain of 40 to 60 residues that coordinates two zinc ions, and has the consensus sequence: C-X2-C-X(9-39)-C-X(1-3)-H-X(2-3)-C-X2-C-X(4-48)-C-X2-C. Many RING finger proteins play a key role in the ubiquitination pathway. A candidate colon cancer tumor suppressor genes identified using nonsense-mediated mRNA decay in colon cancer cells.
Protein type: EC 6.3.2.-; Ligase; Ubiquitin ligase; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 21q22.11
Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity
Biological Process: protein autoubiquitination
Research Articles on LTN1
Similar Products
Product Notes
The LTN1 ltn1 (Catalog #AAA9215022) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1087-1115. The RNF160 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNF160 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the LTN1 ltn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF160, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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