Rabbit anti-Human MRPL48 Polyclonal Antibody | anti-MRPL48 antibody

MRPL48, NT (MRPL48, 39S ribosomal protein L48, mitochondrial) (Biotin)

Cat. Num. AAA6322232

• Gene Names: MRPL48; L48MT; CGI-118; HSPC290; MRP-L48
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MRPL48; Polyclonal Antibody; NT (MRPL48; 39S ribosomal protein L48; mitochondrial) (Biotin); mitochondrial; anti-MRPL48 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MRPL48 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MRPL48 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 30-59 amino acids selected from the N-terminal region of human MRPL48.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MRPL48 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the MRPL48 antibody detected the MRPL48 protein (arrow).)

Related Product Information for anti-MRPL48 antibody

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 6p. [provided by RefSeq].

Product Categories/Family for anti-MRPL48 antibody

Antibodies; Mitochondrial Proteins

References

Fernandez-Ranvier, G.G., et al. World J Surg 32(5):873-881(2008). Lamesch, P., et al. Genomics 89(3):307-315(2007). Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005). Zhang, Z., et al. Genomics 81(5):468-480(2003). Koc, E.C., et al. J. Biol. Chem. 276(47):43958-43969(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 7706333
• NCBI GeneID: 51642
• NCBI Accession #: NP_057139.1
• NCBI GenBank Nucleotide #: NM_016055.5
• UniProt Accession #: Q96GC5; Q49AK7; Q4U2Q4; Q9P091; Q9Y5J0; B4DN34
• Molecular Weight: 23,935 Da
• NCBI Official Full Name: 39S ribosomal protein L48, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein L48
• NCBI Official Symbol: MRPL48
• NCBI Official Synonym Symbols: L48MT; CGI-118; HSPC290; MRP-L48
• NCBI Protein Information: 39S ribosomal protein L48, mitochondrial
• UniProt Protein Name: 39S ribosomal protein L48, mitochondrial
• Protein Family: 39S ribosomal protein
• UniProt Gene Name: MRPL48
• UniProt Synonym Gene Names: L48mt; MRP-L48
• UniProt Entry Name: RM48_HUMAN

Uniprot Description

MRPL48: Interacts with OXA1L.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: mitochondrial inner membrane; mitochondrial large ribosomal subunit; mitochondrial ribosome

Molecular Function: protein binding