Western Blot (WB)
(RNF150 Antibody (N-term) western blot analysis in SiHa cell line lysates (35ug/lane).This demonstrates the RNF150 Antibody detected the RNF150 protein (arrow).)
Rabbit anti-Human, Mouse RNF150 Polyclonal Antibody | anti-RNF150 antibody
RNF150, NT (RNF150, KIAA1214, RING finger protein 150) (Azide free) (HRP)
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
RNF150; Polyclonal Antibody; NT (RNF150; KIAA1214; RING finger protein 150) (Azide free) (HRP); RING finger protein 150; anti-RNF150 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-RNF150 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
RNF150 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-87 amino acids from the N-terminal region of human RNF150.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(RNF150 Antibody (N-term) western blot analysis in SiHa cell line lysates (35ug/lane).This demonstrates the RNF150 Antibody detected the RNF150 protein (arrow).)
Western Blot (WB)
(RNF150 Antibody (N-term) western blot analysis in SiHa cell line lysates (35ug/lane).This demonstrates the RNF150 Antibody detected the RNF150 protein (arrow).)
Western Blot (WB)
(RNF150 Antibody (N-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the RNF150 Antibody detected the RNF150 protein (arrow).)
Western Blot (WB)
(RNF150 Antibody (N-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the RNF150 Antibody detected the RNF150 protein (arrow).)
Related Product Information for anti-RNF150 antibody
RNF150 contains a RING-type zinc finger, a motif known to be involved in protein-protein and protein-DNA interactions. The specific function of this protein is unknown.
Product Categories/Family for anti-RNF150 antibody
References
Rose, J. Phd, et al. Mol. Med. (2010). In press: Hillier, L.W., et al. Nature 434(7034):724-731(2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,834 Da
NCBI Official Full Name
RING finger protein 150
NCBI Official Synonym Full Names
ring finger protein 150
NCBI Official Symbol
RNF150
NCBI Protein Information
RING finger protein 150
UniProt Protein Name
RING finger protein 150
Protein Family
UniProt Gene Name
RNF150
UniProt Synonym Gene Names
KIAA1214
UniProt Entry Name
RN150_HUMAN
Research Articles on RNF150
Similar Products
Product Notes
The RNF150 rnf150 (Catalog #AAA6342669) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNF150, NT (RNF150, KIAA1214, RING finger protein 150) (Azide free) (HRP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RNF150 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RNF150 rnf150 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF150, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
