Rabbit anti-Human Rnasen Polyclonal Antibody | anti-Rnasen antibody

Rnasen, ID (Drosha, Etohi2, Rn3, Rnasen, Ribonuclease 3, Protein Drosha, Ribonuclease III) (AP)

Cat. Num. AAA6342489

• Gene Names: Drosha; Rn3; Etohi2; Rnasen; AI874853; 1110013A17Rik
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Rnasen; Polyclonal Antibody; ID (Drosha; Etohi2; Rn3; Ribonuclease 3; Protein Drosha; Ribonuclease III) (AP); Drosha; Ribonuclease III; anti-Rnasen antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-Rnasen antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Rnasen antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-764 amino acids from the Central region of mouse Rnasen.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Rnasen Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Rnasen antibody detected the Rnasen protein (arrow).)

Related Product Information for anti-Rnasen antibody

Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies (By similarity).

Product Categories/Family for anti-Rnasen antibody

Antibodies; Enzymes; Ribonuclease

References

Chong, M.M., et al. Genes Dev. 24(17):1951-1960(2010). Yang, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(34):15163-15168(2010). Michon, F., et al. Dev. Biol. 340(2):355-368(2010). Wu, H., et al. PLoS ONE 4 (10), E7566 (2009): Shenoy, A., et al. PLoS ONE 4 (9), E6971 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 194328670
• NCBI GeneID: 14000
• NCBI Accession #: NP_001123621.1
• NCBI GenBank Nucleotide #: NM_001130149.1
• UniProt Accession #: Q5HZJ0
• Molecular Weight: 158,828 Da
• NCBI Official Full Name: ribonuclease 3
• NCBI Official Synonym Full Names: drosha, ribonuclease type III
• NCBI Official Symbol: Drosha
• NCBI Official Synonym Symbols: Rn3; Etohi2; Rnasen; AI874853; 1110013A17Rik
• NCBI Protein Information: ribonuclease 3
• UniProt Protein Name: Ribonuclease 3
• UniProt Gene Name: Drosha
• UniProt Synonym Gene Names: Etohi2; Rn3; Rnasen; RNase III

Uniprot Description

Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.