Rabbit anti-Human RNASEH2A Polyclonal Antibody | anti-RNASEH2A antibody

RNASEH2A (Ribonuclease H2 Subunit A, RNase H2 Subunit A, Ribonuclease HI Subunit A, Ribonuclease HI Large Subunit, RNase HI Large Subunit, RNase H(35), Aicardi-Goutieres Syndrome 4 Protein, AGS4, RNASEHI, RNHIA) (FITC)

Cat. Num. AAA6392631

• Gene Names: RNASEH2A; AGS4; JUNB; RNHL; RNHIA; RNASEHI
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RNASEH2A; Polyclonal Antibody; RNASEH2A (Ribonuclease H2 Subunit A; RNase H2 Subunit A; Ribonuclease HI Subunit A; Ribonuclease HI Large Subunit; RNase HI Large Subunit; RNase H(35); Aicardi-Goutieres Syndrome 4 Protein; AGS4; RNASEHI; RNHIA) (FITC); anti-RNASEH2A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RNASEH2A.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RNASEH2A antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full-length human RNASEH2A, aa1-299 (NP_006388.2).
• Immunogen Sequence: MDLSELERDNTGRCRLSSPVPAVCRKEPCVLGVDEAGRGPVLGPMVYAICYCPLPRLADLEALKVADSKTLLESERERLFAKMEDTDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTATGLIQYALDQGVNVTQVFVDTVGMPETYQARLQQSFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKKWQFVEKLQDLDTDYGSGYPNDPKTKAWLKEHVEPVFGFPQFVRFSWRTAQTILEKEAEDVIWEDSASENQEGLRKITSYFLNEGSQARPRSSHRYFLERGLESATSL
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RNASEH2A rabbit polyclonal antibody. Western Blot analysis of RNASEH2A expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of RNASEH2A expression in transfected 293T cell line by RNASEH2A polyclonal antibody. Lane 1: RNASEH2A transfected lysate (33.4kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-RNASEH2A antibody

Antibodies; Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 38455391
• NCBI GeneID: 10535
• NCBI Accession #: NP_006388.2
• NCBI GenBank Nucleotide #: NM_006397
• UniProt Accession #: O75792; Q96F11; B2RCY1
• Molecular Weight: 33,395 Da
• NCBI Official Full Name: ribonuclease H2 subunit A
• NCBI Official Synonym Full Names: ribonuclease H2, subunit A
• NCBI Official Symbol: RNASEH2A
• NCBI Official Synonym Symbols: AGS4; JUNB; RNHL; RNHIA; RNASEHI
• NCBI Protein Information: ribonuclease H2 subunit A; RNase H(35); RNase H2 subunit A; RNase HI large subunit; aicardi-Goutieres syndrome 4 protein; ribonuclease H2, large subunit; ribonuclease HI large subunit; ribonuclease HI subunit A; ribonuclease HI, large subunit
• UniProt Protein Name: Ribonuclease H2 subunit A
• Protein Family: Ribonuclease
• UniProt Gene Name: RNASEH2A
• UniProt Synonym Gene Names: RNASEHI; RNHIA; RNase H2 subunit A; AGS4; RNase HI large subunit
• UniProt Entry Name: RNH2A_HUMAN

NCBI Description

The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid.[provided by RefSeq, Aug 2009]

Uniprot Description

RNASEH2A: Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging- strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4). A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood. Belongs to the RNase HII family. Eukaryotic subfamily.

Protein type: Ribonuclease; EC 3.1.26.4; DNA replication

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleus

Molecular Function: RNA binding; metal ion binding; ribonuclease H activity; ribonuclease activity

Biological Process: mismatch repair; RNA catabolic process; DNA replication

Disease: Aicardi-goutieres Syndrome 4