Porcine Haptoglobin precursor ELISA Kit | Pre-Hpt elisa kit

Porcine Haptoglobin precursor ELISA Kit

Cat. Num. AAA743714

• Gene Names: HP; BP; HPA1S; HP2ALPHA2
• Reactivity: Porcine
• Synonyms: Haptoglobin precursor; Porcine Haptoglobin precursor ELISA Kit; Pre-Hpt elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This pre-Hpt/HAP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine pre-Hpt/HAP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Pre-Hpt elisa kit

Principle of the Assay: pre-Hpt/HAP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-pre-Hpt/HAP antibody and an pre-Hpt/HAP-HRP conjugate. The assay sample and buffer are incubated together with pre-Hpt/HAP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the pre-Hpt/HAP concentration since pre-Hpt/HAP from samples and pre-Hpt/HAP-HRP conjugate compete for the anti-pre-Hpt/HAP antibody binding site. Since the number of sites is limited, as more sites are occupied by pre-Hpt/HAP from the sample, fewer sites are left to bind pre-Hpt/HAP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The pre-Hpt/HAP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Pre-Hpt elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 386783
• NCBI GeneID: 3240
• Molecular Weight: 38,452 Da
• NCBI Official Full Name: haptoglobin
• NCBI Official Synonym Full Names: haptoglobin
• NCBI Official Symbol: HP
• NCBI Official Synonym Symbols: BP; HPA1S; HP2ALPHA2
• NCBI Protein Information: haptoglobin; zonulin; binding peptide; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; haptoglobin, beta polypeptide; haptoglobin, alpha polypeptide
• UniProt Protein Name: Haptoglobin
• UniProt Gene Name: HP
• UniProt Entry Name: HPT_HUMAN

NCBI Description

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HP: Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. Defects in HP are the cause of anhaptoglobinemia (AHP). AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: extracellular space; extracellular region

Molecular Function: antioxidant activity; protein binding; hemoglobin binding; catalytic activity

Biological Process: receptor-mediated endocytosis; response to hydrogen peroxide; immune system process; metabolic process; negative regulation of oxidoreductase activity; defense response to bacterium; acute-phase response; defense response

Disease: Anhaptoglobinemia