Rabbit anti-Human RNASE3 Polyclonal Antibody | anti-RNASE3 antibody

RNASE3, CT (RNASE3, ECP, RNS3, Eosinophil cationic protein, Ribonuclease 3) (AP)

Cat. Num. AAA6342412

• Gene Names: RNASE3; ECP; RNS3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RNASE3; Polyclonal Antibody; CT (RNASE3; ECP; RNS3; Eosinophil cationic protein; Ribonuclease 3) (AP); Ribonuclease 3; anti-RNASE3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RNASE3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-RNASE3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 124-151 in the C-terminal region of human RNASE3, UniProt Accession #P12724.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H460 cell line lysates using MBS642609 (35ug/lane). This demonstrates the RNASE3 antibody detected the RNASE3 protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of MBS642609 with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-RNASE3 antibody

Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity.

Product Categories/Family for anti-RNASE3 antibody

Antibodies; Enzymes; Ribonuclease

References

1. Peona, V., et al. J Asthma 47(2):131-134(2010). 2. Kang, I., et al. Eur Arch Otorhinolaryngol 267(3):391-395(2010). 3. Jonsson, U.B., et al. BMC Pulm Med 10, 36 (2010). 4. Kato, M., et al. Int. Arch. Allergy Immunol. 152 SUPPL 1, 62-66 (2010). 5. Chang, K.C., et al. BMC Cell Biol. 11, 6 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 45243507
• NCBI GeneID: 6037
• NCBI Accession #: NP_002926.2
• NCBI GenBank Nucleotide #: NM_002935.2
• UniProt Accession #: P12724; Q4VBC1; Q8WTP7; Q8WZ62; Q9GZN9
• Molecular Weight: 18,385 Da
• NCBI Official Full Name: eosinophil cationic protein
• NCBI Official Synonym Full Names: ribonuclease, RNase A family, 3
• NCBI Official Symbol: RNASE3
• NCBI Official Synonym Symbols: ECP; RNS3
• NCBI Protein Information: eosinophil cationic protein; RNase 3; ribonuclease 3; cytotoxic ribonuclease
• UniProt Protein Name: Eosinophil cationic protein
• Protein Family: Eosinophil cationic protein
• UniProt Gene Name: RNASE3
• UniProt Synonym Gene Names: ECP; RNS3; ECP; RNase 3
• UniProt Entry Name: ECP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014]

Uniprot Description

RNASE3: Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. Belongs to the pancreatic ribonuclease family.

Protein type: Secreted, signal peptide; Ribonuclease; EC 3.1.27.-; Secreted

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular space; extracellular region

Molecular Function: nucleic acid binding; endonuclease activity; ribonuclease activity

Biological Process: defense response to Gram-positive bacterium; innate immune response in mucosa; RNA catabolic process; antibacterial humoral response