Rabbit RNA Polymerase II, Rpb1 subunit CTD Polyclonal Antibody | anti-RpII215 antibody

RNA Polymerase II, Rpb1 subunit, phosphorylated CTD (Ser2/5) (Rpb1, Pol2)

Cat. Num. AAA614614

• Gene Names: RpII215; 5; 8WG16; CG1554; CTD; DmelCG1554; dRpb1; dRPB1; H5; II; l(1)10Ca; l(1)DC912; l(1)DF912; l(1)G0040; l(1)L5; L5; POL; pol II; Pol II; Pol II CTD; Pol II Ser5P; Pol II0[ser2]; Pol II0[ser5]; Pol IIa; Pol IIo; Pol IIo[ser2]; Pol IIo[Ser2]; Pol IIo[ser5]; Pol
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Puriifed by Protein A and peptide affinity chromatography.
• Synonyms: RNA Polymerase II; Rpb1 subunit CTD; Polyclonal Antibody; Rpb1 subunit; phosphorylated CTD (Ser2/5) (Rpb1; Pol2); Anti -RNA Polymerase II; anti-RpII215 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Detects endogenous levels of Rpb1 only when the carboxy-termial domain heptapeptide repeat [Tyr1, Ser2, Pro3, Thr4, Ser5, Pro6, Ser7] is phosphorylated at serine 2/5. Species Crossreactivity
• Purity/Purification: Affinity Purified; Puriifed by Protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-RpII215 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:1000; Immunohistochemistry (Paraffin): 1:25 (Citrate/TBST)
• Immunogen: Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser2/5 of the Rpb1 CTD heptapeptide.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extract Western Blot analysis of extracts from MCF-7 cells, untreated or treated with doxorubicin (0.5uM, 36h), doxorubicin followed by Lambda Phosphatase (10,000Units/ml, 1h), nocodazole (50ng/ml, 36h) or nocodazole followed by Lambda Phosphatase, using MBS614614 (upper) or p53 Antibody (lower).s from MCF-7 cells, untreated or treated with doxorubicin (0.5 uM, 36h), doxorubicin followed by Lambda Phosphatase (10,000 Units/ml, 1h), nocodazole (50ng/ml, 36h) or nocodazole followed by Lambda Phosphatase, using MBS614614 (upper) or p53 Antibody (lower).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing nuclear localization, using MBS614614.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MBS614614 in the presence of control peptide (left) or antigen-specific peptide (right).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, untreated (left) or lambda phosphatase-treated (right), using MBS614614.)

Related Product Information for anti-RpII215 antibody

RNA polymerase II is a complex of 12 proteins that participates in transcription, mRNA processing, and transcription-coupled DNA repair (1,2). The largest subunit, Rpb1, contains a unique heptapeptide: Tyr1, Ser2, Pro3, Thr4, Ser5, Pro6, Ser7, which is repeated up to 52 times in the carboxy-terminal domain CTD) of Rpb1 (3). This CTD is phosphorylated by cyclin-dependent kinases (cdks), p44/42 MAPK, DNAPKcs, and c-Abl (4). DNA damage caused by UV-light, hydrogen peroxide, or cisplatin results in ubiquitination and proteasomal degradation of Rpb1 (1,3). The kinase inhibitor 5, 6-dichloro-1-b- D-ribofuranosylbenzimidazole prevents ubiquitination of Rpb1, suggesting that CTD phosphorylation is required for proteolysis (5).

Product Categories/Family for anti-RpII215 antibody

Antibodies; Abs to Enzymes; Polymerase

NCBI and Uniprot Product Information

• NCBI GI #: 17530899
• NCBI GeneID: 32100
• NCBI Accession #: NP_511124.1
• NCBI GenBank Nucleotide #: NM_078569.2
• UniProt Accession #: P04052; Q9VYX6
• Molecular Weight: 209,168 Da
• NCBI Official Full Name: RNA polymerase II 215kD subunit
• NCBI Official Synonym Full Names: RNA polymerase II 215kD subunit
• NCBI Official Symbol: RpII215
• NCBI Official Synonym Symbols: 5; 8WG16; CG1554; CTD; DmelCG1554; dRpb1; dRPB1; H5; II; l(1)10Ca; l(1)DC912; l(1)DF912; l(1)G0040; l(1)L5; L5; POL; pol II; Pol II; Pol II CTD; Pol II Ser5P; Pol II0[ser2]; Pol II0[ser5]; Pol IIa; Pol IIo; Pol IIo[ser2]; Pol IIo[Ser2]; Pol IIo[ser5]; Pol
• NCBI Protein Information: CG1554 gene product from transcript CG1554-RA; CG1554-PA; Pol II CTD; Pol II large subunit; RNA Pol II; RNA polymerase II; RNA polymerase II 215kD subunit; RNA polymerase II large subunit; RNA polymerase IIo; RNA polymeraseII-215 kd subunit; RNA-polymerase II; RpII215-PA; Ultrabithorax-like; polymerase II CTD
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB1
• UniProt Gene Name: RpII215
• UniProt Synonym Gene Names: RNA polymerase II subunit B1
• UniProt Entry Name: RPB1_DROME

Uniprot Description

Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing

By similarity.

Catalytic activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure: Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits

By similarity.

Subcellular location: Nucleus

By similarity.

Post-translational modification: The tandem 7 residues repeats in the C-terminal domain (CTD) can be highly phosphorylated. The phosphorylation activates Pol II. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the heptapepdtide repeat. The phosphorylation state is believed to result from the balanced action of site-specific CTD kinases and phosphatase, and a 'CTD code' that specifies the position of Pol II within the transcription cycle has been proposed.

Miscellaneous: The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion temporarily coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits. The ribonucleoside triphosphate is transferred by a rotation to the nucleotide addition (A) site for pairing with the template DNA. The catalytic A site involves three conserved aspartate residues of the RNA Pol II largest subunit which permanently coordinate a second magnesium ion.

Sequence similarities: Belongs to the RNA polymerase beta' chain family.