Rabbit RKHD3 Polyclonal Antibody | anti-MEX3B antibody

RKHD3 Antibody

Cat. Num. AAA150066

• Gene Names: MEX3B; RKHD3; MEX-3B; RNF195
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: RKHD3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: RKHD3; Polyclonal Antibody; RKHD3 Antibody; MEX-3B; RNF195; KIAA2009; RNA-binding protein MEX3B; RING finger and KH domain-containing protein 3; mex-3 homolog B; anti-MEX3B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody is specific for RKHD3 and will not recognize the other RKHD3 family of proteins.
• Purity/Purification: RKHD3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 569

• Applicable Applications for anti-MEX3B antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: RKHD3 antibody can be used for detection of EPAC1 by Western blot at 1 mug/mL. Antibody can also be used for immunofluorescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: RKHD3 antibody was raised against a 16 amino acid synthetic peptide from near the center of human RKHD3.
• Buffer: RKHD3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: RKHD3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of RKHD3 in human testis tissue lysate with RKHD3 antibody at 1 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of RKHD3 in mouse skeletal muscle cells with RKHD3 antibody at 20 μg/mL.)

Related Product Information for anti-MEX3B antibody

RKHD3 Antibody: Rkhd3, also known as MEX3B is a member of a novel family of four homologous human MEX3 proteins each containing two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one carboxy-terminal RING finger module. MEX3 proteins, including Rkhd3, are phosphoproteins that bind RNA through their KH domains and shuttle between the nucleus and the cytoplasm via the CRM1 export pathway. These proteins are a novel family of evolutionarily conserved RNA-binding proteins, differentially recruited to P bodies and potentially involved in post-transcriptional regulatory mechanisms. Rkhd3 is thought to act as a mechanism to fine-tune mRNA regulation in early Xenopus development, and with Rkhd4, but not Rkhd1, will co-localize with both the hDcp1a decapping factor and Argonaute (Ago) proteins in processing bodies (P bodies), recently characterized as centers of mRNA turnover.

NCBI and Uniprot Product Information

• NCBI GI #: 47716512
• NCBI GeneID: 84206
• NCBI Accession #: NP_780575
• NCBI GenBank Nucleotide #: NM_032246.4
• UniProt Accession #: Q6ZN04; Q4G0W1; Q8IVG2; Q9H0J0
• Molecular Weight: 16,780 Da
• NCBI Official Full Name: RNA-binding protein MEX3B
• NCBI Official Synonym Full Names: mex-3 RNA binding family member B
• NCBI Official Symbol: MEX3B
• NCBI Official Synonym Symbols: RKHD3; MEX-3B; RNF195
• NCBI Protein Information: RNA-binding protein MEX3B; RING finger protein 195; ring finger and KH domain containing 3
• UniProt Protein Name: RNA-binding protein MEX3B
• Protein Family: RNA-binding protein
• UniProt Gene Name: MEX3B
• UniProt Synonym Gene Names: KIAA2009; RKHD3; RNF195
• UniProt Entry Name: MEX3B_HUMAN

NCBI Description

This gene encodes an RNA-binding phosphoprotein that is part of the MEX3 (muscle excess 3) family of translational regulators. The encoded protein contains N-terminal nuclear export and nuclear localization signals and is exported from the cytoplasm to the nucleus. The protein binds to RNA via two KH domains and also colocalizes with MEX3A, Dcp1A decapping factor and Argonaute proteins within P (processing) bodies. [provided by RefSeq, Oct 2012]

Uniprot Description

RKHD3: RNA-binding protein. May be involved in post- transcriptional regulatory mechanisms. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 15q25.2

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: zinc ion binding; calcium ion binding

Biological Process: protein amino acid autophosphorylation; protein amino acid phosphorylation