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Western Blot (WB) (Western blot analysis of anti-ANKRD3-Q817 Pab in mouse kidney tissue lysate (35ug/lane). ANKRD3-Q817(arrow) was detected using the purified Pab.)

Rabbit anti-Human, mouse RIPK4 (ANKRD3) Polyclonal Antibody | anti-RIPK4 antibody

RIPK4 (ANKRD3) Antibody (C-term)

Reactivity
Human, mouse
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RIPK4 (ANKRD3); Polyclonal Antibody; RIPK4 (ANKRD3) Antibody (C-term); Receptor-interacting serine/threonine-protein kinase 4; Ankyrin repeat domain-containing protein 3; PKC-delta-interacting protein kinase; RIPK4; ANKRD3; DIK; anti-RIPK4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RIPK4 (ANKRD3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 802-832 amino acids from the C-terminal region of human RIPK4 (ANKRD3).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
802-832
Sequence Length
784
Applicable Applications for anti-RIPK4 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-ANKRD3-Q817 Pab in mouse kidney tissue lysate (35ug/lane). ANKRD3-Q817(arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of anti-ANKRD3-Q817 Pab in mouse kidney tissue lysate (35ug/lane). ANKRD3-Q817(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(ANKRD3 Antibody (Q817) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ANKRD3 antibody detected the ANKRD3 protein (arrow).)

Western Blot (WB) (ANKRD3 Antibody (Q817) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ANKRD3 antibody detected the ANKRD3 protein (arrow).)
Related Product Information for anti-RIPK4 antibody
The protein encoded by this gene is a serine/threonine protein kinase that interacts with protein kinase C-delta. The encoded protein can also activate NFkappaB and is required for keratinocyte differentiation. This kinase undergoes autophosphorylation.
Product Categories/Family for anti-RIPK4 antibody
References
Hattori, M., et al., Nature 405(6784):311-319 (2000).

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
91611
NCBI Official Full Name
Receptor-interacting serine/threonine-protein kinase 4
UniProt Protein Name
Receptor-interacting serine/threonine-protein kinase 4
UniProt Gene Name
RIPK4
UniProt Synonym Gene Names
ANKRD3; DIK
UniProt Entry Name
RIPK4_HUMAN

Uniprot Description

ANKRD3: a TKL protein kinase of the RIPK family. Activates NFkappaB when overexpressed in cell lines and is required for keratinocyte differentiation in vivo. Appears to activate NFkappaB in both a kinase-dependent as well as a kinase-independent manner. Contains 10 ANK repeats. Two alternatively spliced isoforms have been described.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, TKL; TKL group; RIPK family

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: membrane; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: morphogenesis of an epithelium; protein amino acid phosphorylation; activation of NF-kappaB transcription factor

Disease: Popliteal Pterygium Syndrome, Lethal Type

Similar Products

Product Notes

The RIPK4 ripk4 (Catalog #AAA9214691) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 802-832. The RIPK4 (ANKRD3) Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RIPK4 (ANKRD3) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the RIPK4 ripk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RIPK4 (ANKRD3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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