Rabbit RIPK1 Polyclonal Antibody | anti-RIPK1 antibody

RIPK1 Antibody

Cat. Num. AAA150077

• Gene Names: RIPK1; RIP; RIP1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: RIPK1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: RIPK1; Polyclonal Antibody; RIPK1 Antibody; RIP; RIP1; Cell death protein RIP; RIP-1; receptor (TNFRSF)-interacting serine-threonine kinase 1; anti-RIPK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: RIPK1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 671

• Applicable Applications for anti-RIPK1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: RIPK1 antibody can be used for detection of RIPK1 by Western blot at 1 ?g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ?g/mL. For immunofluorescence start at 20 ?g/mL.; ; Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Conjugate: Unconjugated
• Immunogen: RIPK1 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human RIPK1.
• Buffer: RIPK1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Positive Control: 1) Cat. No. MBS151605 - Rat Kidney Tissue Lysate
• Preparation and Storage: RIPK1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of RIPK1 in rat kidney tissue lysate with RIPK1 antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of RIPK1 in mouse kidney tissue with RIPK1 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of RIPK1 in Mouse Kidney cells with RIPK1 antibody at 20 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of RIPK1 in mouse kidney tissue with RIPK1 antibody at 2.5 μg/ml.)

Related Product Information for anti-RIPK1 antibody

RIPK1 Antibody: RIPK1 (Receptor Interacting Protein) is a crucial 74 kD adaptor kinase in several of stress-induced signaling pathways and on the crossroad of a cell's decision to live or die. RIPK1 contains an N-terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C-terminal region containing a death domain motif present in the Fas and TNFR1 intracellular domains. Full length RIPK1 is important for signallling to NFκ-B, MAPKs and necrosis, whereas caspase-8 generates a C-terminal RIPK1 cleavage fragment, promoting TNF-induced apoptosis. It is required for TNFRSF1A-mediated and TLR3-induced NF-κB activation. RIPK1-deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age.

NCBI and Uniprot Product Information

• NCBI GI #: 57242761
• NCBI GeneID: 8737
• NCBI Accession #: NP_003795
• NCBI GenBank Nucleotide #: NM_003804.3
• UniProt Accession #: Q13546; Q13180; Q59H33; A0AV89; B2RAG1; B4E3F9
• NCBI Official Full Name: receptor-interacting serine/threonine-protein kinase 1
• NCBI Official Synonym Full Names: receptor (TNFRSF)-interacting serine-threonine kinase 1
• NCBI Official Symbol: RIPK1
• NCBI Official Synonym Symbols: RIP; RIP1
• NCBI Protein Information: receptor-interacting serine/threonine-protein kinase 1; RIP-1; cell death protein RIP; receptor interacting protein; receptor-interacting protein 1; serine/threonine-protein kinase RIP
• UniProt Protein Name: Receptor-interacting serine/threonine-protein kinase 1
• Protein Family: Receptor-interacting serine/threonine-protein kinase
• UniProt Gene Name: RIPK1
• UniProt Synonym Gene Names: RIP; RIP1; RIP-1
• UniProt Entry Name: RIPK1_HUMAN

Uniprot Description

RIPK1: Serine-threonine kinase which transduces inflammatory and cell-death signals (necroptosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necroptosis-inducing complex. Interacts (via RIP homotypic interaction motif) with RIPK3 (via RIP homotypic interaction motif); this interaction induces RIPK1 necroptosis-specific phosphorylation, formation of the necroptosis-inducing complex. Interacts (via the death domain) with TNFRSF6 (via the death domain) and TRADD (via the death domain). Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RNF216, EGFR, IKBKG, TRAF1, TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interact with MAVS/IPS1. Interacts with ZFAND5. Interacts with RBCK1. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Inhibited by necrostatin-1. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, TKL; TKL group; RIPK family

Chromosomal Location of Human Ortholog: 6p25.2

Cellular Component: mitochondrion; cytoplasm; cytosol; receptor complex; lipid raft

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; death receptor binding; protein complex binding; ATP binding; protein kinase activity

Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein heterooligomerization; positive regulation of apoptosis; apoptosis; MyD88-independent toll-like receptor signaling pathway; protein amino acid autophosphorylation; positive regulation of JNK cascade; negative regulation of I-kappaB kinase/NF-kappaB cascade; toll-like receptor 3 signaling pathway; positive regulation of tumor necrosis factor production; activation of JNK activity; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; positive regulation of programmed cell death; tumor necrosis factor-mediated signaling pathway; cellular protein catabolic process; toll-like receptor signaling pathway; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; positive regulation of macrophage differentiation; toll-like receptor 4 signaling pathway; protein homooligomerization