Western Blot (WB)
(Western blot analysis of whole cell lysate from mouse 3T3 cells probed with in the absence (A) or presence (B) of blocking peptide)
Rabbit anti-Mouse, Rat RIP3 Polyclonal Antibody | anti-RIP3 antibody
RIP3 (Receptor-interacting Serine/Threonine-protein Kinase 3, RIP-like Protein Kinase 3, RIPK3, Receptor-interacting Protein 3, RIP-3, mRIP3) (MaxLight 550)
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Purified by affinity chromatography.
Synonyms
RIP3; Polyclonal Antibody; RIP3 (Receptor-interacting Serine/Threonine-protein Kinase 3; RIP-like Protein Kinase 3; RIPK3; Receptor-interacting Protein 3; RIP-3; mRIP3) (MaxLight 550); Receptor-interacting Serine-threonine Kinase 3; 2610528K09Rik; AW107945; EC=2.7.11.1; anti-RIP3 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse RIP3. Species crossreactivity: rat.
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight550.
Applicable Applications for anti-RIP3 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
WB: 0.5-1ug/ml detects a band of ~56kD in Mouse 3T3 whole cell lysate
IHC-P: 5ug/ml. Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Applications are based on unconjugated antibody.
IHC-P: 5ug/ml. Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide sequence corresponding to aa473-486 of mouse RIP3.
Conjugate
MaxLight550
Note
Preservative Free
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.
Western Blot (WB)
(Western blot analysis of whole cell lysate from mouse 3T3 cells probed with in the absence (A) or presence (B) of blocking peptide)
Western Blot (WB)
(Western blot analysis of whole cell lysate from mouse 3T3 cells probed with in the absence (A) or presence (B) of blocking peptide)
Immunohistochemistry (IHC)
(IHC staining of rat kidney withMBS617965.)
Immunohistochemistry (IHC)
(IHC staining of rat kidney withMBS617965.)
Product Categories/Family for anti-RIP3 antibody
References
1. Yu, P.W., et al (1999). Identification of RIP3, a RIP-like kinase that activates apoptosis and NF?B. Curr. Biol. 9:539-42 2. Sun, X., et al (1999). RIP3, a novel apoptosis-inducing kinase. J Biol Chem. 274:16871-5. 3. Pazdernik, N.J., et al (1999). Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-?B. Mol Cell Bio.19:6500-8.
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Product Notes
The RIP3 (Catalog #AAA6497924) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RIP3 (Receptor-interacting Serine/Threonine-protein Kinase 3, RIP-like Protein Kinase 3, RIPK3, Receptor-interacting Protein 3, RIP-3, mRIP3) (MaxLight 550) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RIP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. WB: 0.5-1ug/ml detects a band of ~56kD in Mouse 3T3 whole cell lysate IHC-P: 5ug/ml. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RIP3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RIP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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