SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
CLPP recombinant protein
CLPP, 57-277aa, Human, E Coli
Gene Names
CLPP; DFNB81; PRLTS3
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
CLPP; 57-277aa; Human; E Coli; Putative ATP-dependent Clp protease proteolytic subunit mitochondrial; Endopeptidase Clp; CLPP recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl buffer (pH 7.5) containing 2mM DTT, 20% glycerol, 100mM NaCl
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence Positions
57-277aa
Sequence
MPLIPIVVEQ TGRGERAYDI YSRLLRERIV CVMGPIDDSV ASLVIAQLLF LQSESNKKPI HMYINSPGGV VTAGLAYDT MQYILNPICT WCVGQAASMG SLLLAAGTPG MRHSLPNSRI MIHQPSGGAR GQATDIAIQA EEIMKLKKQL YNIYAKHTKQ SLQVIESAME RDRYMSPMEA QEFGILDKVL VHPPQDGEDE PTLVQKEPVE AAPPAAEPVPA ST
Applicable Applications for CLPP recombinant protein
SDS-PAGE
Preparation and Storage
Can be stored at 2-8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
Related Product Information for CLPP recombinant protein
Background/Introduction: CLPP (ATP-dependent Clp protease proteolytic subunit) belongs to the peptidase family S14. CLPP cleaves peptides in various proteins in a process that requires ATP hydrolysis. CLPP, the catalytic core of the Clp proteolytic complex, is widely involved in many cellular processes via the regulation of intracellular protein quality. CLPP may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. Recombinant human CLPP protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Categories/Family for CLPP recombinant protein
References
Li XH., et al (2010) BMC Microbiol. 19;10:54.; Kang, S.G., et al (2002) J. Biol. Chem. 277: 21095-21102.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24.2kDa (222aa), confirmed by MALDI-TOF
NCBI Official Full Name
ATP-dependent Clp protease proteolytic subunit, mitochondrial
NCBI Official Synonym Full Names
caseinolytic mitochondrial matrix peptidase proteolytic subunit
NCBI Official Symbol
CLPP
NCBI Official Synonym Symbols
DFNB81; PRLTS3
NCBI Protein Information
ATP-dependent Clp protease proteolytic subunit, mitochondrial
UniProt Protein Name
ATP-dependent Clp protease proteolytic subunit, mitochondrial
Protein Family
UniProt Gene Name
CLPP
NCBI Description
The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane. [provided by RefSeq, Jul 2008]
Uniprot Description
Protease component of the Clp complex that cleaves peptides and various proteins in an ATP-dependent process. Has low peptidase activity in the absence of CLPX. The Clp complex can degrade CSN1S1, CSN2 and CSN3, as well as synthetic peptides (in vitro) and may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates.
Research Articles on CLPP
Similar Products
Product Notes
The CLPP clpp (Catalog #AAA204060) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 57-277aa. AAA Biotech's CLPP can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the CLPP clpp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MPLIPIVVEQ TGRGERAYDI YSRLLRERIV CVMGPIDDSV ASLVIAQLLF LQSESNKKPI HMYINSPGGV VTAGLAYDT MQYILNPICT WCVGQAASMG SLLLAAGTPG MRHSLPNSRI MIHQPSGGAR GQATDIAIQA EEIMKLKKQL YNIYAKHTKQ SLQVIESAME RDRYMSPMEA QEFGILDKVL VHPPQDGEDE PTLVQKEPVE AAPPAAEPVP A ST. It is sometimes possible for the material contained within the vial of "CLPP, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
