Rabbit anti-Human Rin Polyclonal Antibody | anti-Rin antibody

Rin

Cat. Num. AAA395588

• Reactivity: Human
• Applications: Western Blot
• Purity: Ammonium Sulfate Precipitation
• Synonyms: Rin; Polyclonal Antibody; anti-Rin antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Ammonium Sulfate Precipitation
• Form/Format: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Sequence Length: 783

• Applicable Applications for anti-Rin antibody: Western Blot (WB)
• Immunogen: His-tagged recombinant human Rin protein
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Related Product Information for anti-Rin antibody

Rit and its neuron-specific homologue, Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64% amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100% conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.

Product Categories/Family for anti-Rin antibody

Polyclonal Antibody; G Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 68989256
• NCBI GeneID: 9610
• NCBI Accession #: NP_004283.2
• NCBI GenBank Nucleotide #: NM_004292.2
• UniProt Accession #: Q13671; O15010; Q00427; Q96CC8
• Molecular Weight: 36
• NCBI Official Full Name: ras and Rab interactor 1
• NCBI Official Synonym Full Names: Ras and Rab interactor 1
• NCBI Official Symbol: RIN1
• NCBI Protein Information: ras and Rab interactor 1; ras inhibitor 1; ras inhibitor JC99; ras inhibitor RIN1; ras interaction/interference protein 1
• UniProt Protein Name: Ras and Rab interactor 1
• Protein Family: Ringhalexin
• UniProt Gene Name: RIN1
• UniProt Entry Name: RIN1_HUMAN

Uniprot Description

Function: Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF) for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis. Ref.3 Ref.5 Ref.8

Subunit structure: Interacts with the GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). This interaction prevents the association between RAF1 and Ras. Interacts with 14-3-3 proteins YWHAB, YWHAE and YWHAZ when phosphorylated on Ser-351. Interacts with the SH3 domain of ABL1 and ABL2. Interacts with RAB5A. The interaction with Ras is probably regulated and antagonized by the interaction with 14-3-3 proteins. The interaction with 14-3-3 proteins is regulated by phosphorylation on Ser-351. Ref.5 Ref.8

Subcellular location: Cytoplasm. Membrane. Cytoplasm › cytoskeleton. Note: Some amount is membrane-associated. Ref.7

Tissue specificity: Expressed in all tissues examined with high levels in brain, placenta and pancreas. Ref.3

Post-translational modification: Phosphorylated on tyrosine residues by ABL1 and ABL2. Phosphorylation at Ser-351 by PRKD1 induces interaction with 14-3-3 proteins. Ref.3 Ref.5 Ref.7 Ref.8

Sequence similarities: Belongs to the RIN (Ras interaction/interference) family.Contains 1 Ras-associating domain.Contains 1 SH2 domain.Contains 1 VPS9 domain.