NFkappaB-p65 Antibody | anti-RELA antibody

NFkappaB-p65 (Ab-536) Antibody

Cat. Num. AAA857015

• Gene Names: RELA; p65; NFKB3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: NFkappaB-p65; NFkappaB-p65 (Ab-536) Antibody; anti-RELA antibody

• Reactivity: Human, Mouse, Rat
• Specificity: NF-kappaB p65 (Ab-536) antibody detects endogenous levels of total NF-kappaB p65 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 548

• Applicable Applications for anti-RELA antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human NF-kappaB p65 around the phosphorylation site of serine 536 (F-S-SP-I-A).
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-kappaB p65 (Ab-536) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells using NF-kappaB p65 (Ab-536) antibody.)

Product Categories/Family for anti-RELA antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Doyle S L, et al. (2005) J Biol Chem. 280(25): 23496-23501. Anwar K N, et al. (2004) J Immunol. 173(11): 6965-6972. Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20. Haskill S, et al. (1991) Cell 65:1281-1289.

NCBI and Uniprot Product Information

• NCBI GI #: 223468681
• NCBI GeneID: 5970
• NCBI Accession #: NP_001138610.1
• NCBI GenBank Nucleotide #: NM_001145138.1
• UniProt Accession #: Q04206; Q6GTV1; Q6SLK1
• Molecular Weight: 59,910 Da
• NCBI Official Full Name: transcription factor p65 isoform 2
• NCBI Official Synonym Full Names: RELA proto-oncogene, NF-kB subunit
• NCBI Official Symbol: RELA
• NCBI Official Synonym Symbols: p65; NFKB3
• NCBI Protein Information: transcription factor p65
• UniProt Protein Name: Transcription factor p65
• Protein Family: Transcription factor
• UniProt Gene Name: RELA
• UniProt Synonym Gene Names: NFKB3

NCBI Description

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681).