Rabbit Rim2 Polyclonal Antibody | anti-RIMS2 antibody

Rim2 Antibody

Cat. Num. AAA150299

• Gene Names: RIMS2; OBOE; RIM2; RAB3IP3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Rim2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: Rim2; Polyclonal Antibody; Rim2 Antibody; OBOE; RIM2; RAB3IP3; KIAA0751; Regulating synaptic membrane exocytosis protein 2; Rab-3-interacting molecule 2; RIM 2; regulating synaptic membrane exocytosis 2; anti-RIMS2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody is predicted to have no cross-reactivity to other Rim proteins.
• Purity/Purification: Rim2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1349

• Applicable Applications for anti-RIMS2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Rim2 antibody can be used for detection of Rim2 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Rim2 antibody was raised against a 17 amino acid synthetic peptide near the center of the human Rim2.
• Buffer: Rim2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Rim2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Rim2 in human brain tissue lysate with Rim2 antibody at 1 μg/mL. )

Immunohistochemistry (IHC)

(Immunohistochemistry of Rim2 in human brain with Rim2 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Rim2 in Human Brain tissue with Rim2 antibody at 20 μg/mL.)

Related Product Information for anti-RIMS2 antibody

Rim2 Antibody: Rab3-interacting molecules (RIMs) are synaptic proteins necessary for neuronal transmission and plasticity. Rim1 and Rim2 proteins are expressed in overlapping but distinct patterns throughout the brain. While the ablation of either gene was not lethal in mice, the deletion of both resulted in postnatal mortality. This lethality is due to a defect in neurotransmitter release; synapses without RIM proteins can still release neurotransmitters but are unable to do so in response to normal Ca2+ triggers. Like Rim1, Rim 2 is thought to be an effector protein for Rab3, binding to Rab3 on synaptic vesicles in a GTP-dependent manner. Rim2 is known to exist in multiple isoforms; this antibody should recognize most of them.

NCBI and Uniprot Product Information

• NCBI GI #: 154354983
• NCBI GeneID: 9699
• NCBI Accession #: NP_001093587
• NCBI GenBank Nucleotide #: NM_001100117.2
• UniProt Accession #: Q9UQ26; O43413; Q86XL9; Q8IWV9; Q8IWW1; B3KX91; F8WD47
• Molecular Weight: 153,363 Da
• NCBI Official Full Name: regulating synaptic membrane exocytosis protein 2 isoform a
• NCBI Official Synonym Full Names: regulating synaptic membrane exocytosis 2
• NCBI Official Symbol: RIMS2
• NCBI Official Synonym Symbols: OBOE; RIM2; RAB3IP3
• NCBI Protein Information: regulating synaptic membrane exocytosis protein 2; RIM 2; nuclear protein; Rab3-interacting protein; RAB3 interacting protein 3; rab-3-interacting protein 3; rab3-interacting molecule 2; rab-3-interacting molecule 2; non-small cell lung cancer RimL3a protein; non-small cell lung cancer RimL3c protein
• UniProt Protein Name: Regulating synaptic membrane exocytosis protein 2
• Protein Family: Regulating synaptic membrane exocytosis protein
• UniProt Gene Name: RIMS2
• UniProt Synonym Gene Names: KIAA0751; RAB3IP3; RIM2; RIM 2
• UniProt Entry Name: RIMS2_HUMAN

Uniprot Description

RIMS2: Rab effector involved in exocytosis. May act as scaffold protein. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 8q22.3

Cellular Component: presynaptic membrane; presynaptic active zone; cell junction

Molecular Function: protein binding; metal ion binding; Rab GTPase binding

Biological Process: regulation of exocytosis; intracellular protein transport; regulation of membrane potential; cAMP-mediated signaling; insulin secretion; calcium ion-dependent exocytosis of neurotransmitter; calcium ion-dependent exocytosis