Rabbit anti-Human SP1 Monoclonal Antibody | anti-SP1 antibody

SP1 Rabbit mAb

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Affinity purification
• Synonyms: SP1; Monoclonal Antibody; SP1 Rabbit mAb; anti-SP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
• Sequence: TTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHPIQGLPLAIANAPGDHGAQLGLHGAGGDGIHDDTAGGEEGENSPDAQPQAGRRTR

• Applicable Applications for anti-SP1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation (ChIP)-seq, CUT&Tag
• Application Notes: WB: 1:1000-1:2000; IHC-P: 1:200-1:2000; IF/ICC: 1:200-1:1000; ELISA: Recommended starting concentration is 1ug/mL.; Please optimize the concentration based on your specific assay requirements.; ChIP: 5ug antibody for 10ug-15ug of Chromatin; ChIP-seq: 1:50-1:100; CUT&Tag: 10? cells /1 ug
• Cross Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 500-600 of human SP1 (P08047).
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

ChIP (Chromatin Immunoprecipitation)

(Chromatin immunoprecipitation analysis of extracts of 293T cells, using SP1 antibody (AAA28521) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

ICC (Immunocytochemistry)

(Confocal imaging of C6 cells using SP1 Rabbit mAb (AAA28521,at dilution of 1:100) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.)

IF (Immunofluorescence)

(Immunofluorescence analysis of NIH-3T3 cells using SP1 Rabbit mAb (AAA28521) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using SP1 Rabbit mAb (AAA28521) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry)

(Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using SP1 Rabbit mAb (AAA28521) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using SP1 Rabbit mAb (AAA28521) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using SP1 Rabbit mAb (AAA28521) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry)

(Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using SP1 Rabbit mAb (AAA28521) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using SP1 Rabbit mAb (AAA28521) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot)

(Western blot analysis of various lysates using SP1 Rabbit mAb (AAA28521) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)

ChIP (Chromatin Immunoprecipitation)

(Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from 293T cells using 5 ug of SP1 Rabbit mAb (AAA28521). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of SP1 in the representative genomic region surrounding TINF2 gene.)

ChIP (Chromatin Immunoprecipitation)

(Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from 293T cells using 5 ug of SP1 Rabbit mAb (AAA28521). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of SP1 across chromosome 14 (upper panel) and the genomic region encompassing TINF2, a representative gene enriched in SP1 (lower panel).)

Application Data

(CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10? Hep G2 cells with 1 ug SP1 Rabbit mAb (AAA28521), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of SP1 in representative gene loci (TINF2), as shown in figure.)

Related Product Information for anti-SP1 antibody

The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-SP1 antibody

Epigenetics Nuclear Signaling; Transcription Factors; Endocrine Metabolism; Mitochondrial metabolism; Nucleotide metabolism; Neuroscience; Neurodegenerative Diseases; Stem Cells; Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 352962149
• NCBI GeneID: 6667
• NCBI Accession #: NP_001238754.1
• NCBI GenBank Nucleotide #: NM_001251825.1
• UniProt Accession #: P08047; Q86TN8; Q9H3Q5; Q9NR51; Q9NY21; Q9NYE7
• Molecular Weight: Calculated MW: 81kDa; Observed MW: 90kDa
• UniProt Protein Name: Transcription factor Sp1
• UniProt Gene Name: SP1
• UniProt Synonym Gene Names: TSFP1
• UniProt Entry Name: SP1_HUMAN