Rabbit anti-Human RICTOR Polyclonal Antibody | anti-RICTOR antibody

Rictor, NT (Rapamycin-insensitive Companion of mTOR, AVO3 Homolog, hAVO3, KIAA1999) (Azide free) (HRP)

Cat. Num. AAA6497789

• Gene Names: RICTOR; PIA; AVO3; hAVO3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RICTOR; Polyclonal Antibody; Rictor; NT (Rapamycin-insensitive Companion of mTOR; AVO3 Homolog; hAVO3; KIAA1999) (Azide free) (HRP); anti-RICTOR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Rictor.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RICTOR antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human Rictor (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Rictor Antibody (N-term) in Ramos cell line lysates (35ug/lane). RICTOR (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human Lymph tissue reacted with Rictor antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-RICTOR antibody

Antibodies; Signal Transduction

References

1. Pearce, L.R., Biochem. J. 405(3), 513-522 (2007). 2. Yang, Q., Genes Dev. 20(20), 2820-2832 (2006). 3. Jacinto, E., Cell 127(1), 125-137 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 59891448
• NCBI GeneID: 253260
• NCBI Accession #: NP_689969
• NCBI GenBank Nucleotide #: NM_152756.3
• UniProt Accession #: Q6R327; Q68DT5; Q86UB7; Q8N3A0; Q8NCM6; B2RNX0; B7ZMF7
• Molecular Weight: 195,011 Da
• NCBI Official Full Name: rapamycin-insensitive companion of mTOR isoform 1
• NCBI Official Synonym Full Names: RPTOR independent companion of MTOR, complex 2
• NCBI Official Symbol: RICTOR
• NCBI Official Synonym Symbols: PIA; AVO3; hAVO3
• NCBI Protein Information: rapamycin-insensitive companion of mTOR; AVO3 homolog; RICTOR; TORC2-specific protein AVO3; pianissimo
• UniProt Protein Name: Rapamycin-insensitive companion of mTOR
• Protein Family: Rapamycin-insensitive companion
• UniProt Gene Name: RICTOR
• UniProt Synonym Gene Names: hAVO3
• UniProt Entry Name: RICTR_HUMAN

Uniprot Description

RICTOR: a regulatory binding partner of the kinase mTOR. Part of a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Rictor interacts with Cullin1-Rbx1 to form an E3 ubiquitin ligase complex, and promotes ubiquitination and degradation of SGK1. Shares homology with pianissimo from D. discoidieum, STE20p from S. pombe, and AVO3p from S. cerevisiae.

Protein type: Cell cycle regulation; Ubiquitin conjugating system; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: TORC2 complex; cytosol

Molecular Function: protein binding; ribosome binding; protein kinase binding

Biological Process: epidermal growth factor receptor signaling pathway; TOR signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; regulation of protein kinase B signaling cascade; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; embryonic development; positive regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; actin cytoskeleton reorganization; innate immune response; positive regulation of endothelial cell proliferation; vascular endothelial growth factor receptor signaling pathway; positive regulation of TOR signaling pathway