Immunofluorescence (IF)
(MBS9602228 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human, Rat Ribosomal Protein S2 Polyclonal Antibody | anti-RPS2 antibody
Ribosomal Protein S2 Antibody
Gene Names
RPS2; S2; LLREP3
Reactivity
Human, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Ribosomal Protein S2; Polyclonal Antibody; Ribosomal Protein S2 Antibody; 40S ribosomal protein S2; 40S ribosomal protein S4; LLREP3; LLRep3 protein; MGC102851; MGC117344; MGC117345; OK/KNS-cl.6; Protein LLRep3; Ribosomal protein S2; rps2; RPS4; RS2; RS2_HUMAN; S2; S4; anti-RPS2 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Ribosomal Protein S2 antibody detects endogenous levels of total Ribosomal Protein S2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
293
Applicable Applications for anti-RPS2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human Ribosomal Protein S2
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (90%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9602228 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9602228 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from rat brain, using Ribosomal Protein S2 Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from rat brain, using Ribosomal Protein S2 Antibody.)
Western Blot (WB)
(Western blot analysis of Ribosomal Protein S2 using HeLa whole cell lysates)
Western Blot (WB)
(Western blot analysis of Ribosomal Protein S2 using HeLa whole cell lysates)
Related Product Information for anti-RPS2 antibody
Post-translational Modifications: Citrullinated by PADI4 in the Arg/Gly-rich region. Asymmetric arginine dimethylation by PRMT3 occurs at multiple sites in the Arg/Gly-rich region.
Similarity: Belongs to the universal ribosomal protein uS5 family.
Similarity: Belongs to the universal ribosomal protein uS5 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 31 kDa
Predicted: 32 kDa
Predicted: 32 kDa
NCBI Official Full Name
40S ribosomal protein S2
NCBI Official Synonym Full Names
ribosomal protein S2
NCBI Official Symbol
RPS2
NCBI Official Synonym Symbols
S2; LLREP3
NCBI Protein Information
40S ribosomal protein S2
UniProt Protein Name
40S ribosomal protein S2
Protein Family
UniProt Gene Name
RPS2
UniProt Synonym Gene Names
RPS4
NCBI Description
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S5P family of ribosomal proteins. It is located in the cytoplasm. This gene shares sequence similarity with mouse LLRep3. It is co-transcribed with the small nucleolar RNA gene U64, which is located in its third intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Research Articles on RPS2
Similar Products
Product Notes
The RPS2 rps2 (Catalog #AAA9602228) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ribosomal Protein S2 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ribosomal Protein S2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the RPS2 rps2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ribosomal Protein S2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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