Rabbit RHOQ Polyclonal Antibody | anti-RHOQ antibody

RHOQ Polyclonal Antibody

Cat. Num. AAA3006479

• Gene Names: RHOQ; ARHQ; TC10; TC10A; RASL7A; HEL-S-42
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RHOQ; Polyclonal Antibody; RHOQ Polyclonal Antibody; Rho-related GTP-binding protein RhoQ; Ras-like protein TC10; Ras-like protein family member 7A; ARHQ; RASL7A; TC10; anti-RHOQ antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RHOQ polyclonal antibody detects endogenous levels of RHOQ protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 205

• Applicable Applications for anti-RHOQ antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human RHOQ.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RHOQ polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)

Related Product Information for anti-RHOQ antibody

tc10 is a small GTP-binding protein that is induced during nerve injury, where it cooperates with other Rho family members to facilitate nerve regeneration and, in particular, neurite elongation. tc10 is located predominantly in the plasma membrane, a property that depends both on its post-translational prenylation and on its ability to bind and hydrolyze GTP. tc10 colocalizes to actin filaments and interacts with the actin-binding and filament-forming protein, profilin. tc10 functions to regulate cellular signaling to the actin cytoskeleton and processes associated with cell growth. tc10 also interacts with a similar subset of effectors for Cdc42 and is regulated differentially by p50Rho GTPase-activating protein. Activated tc10 interacts with a variety of putative Rho family effectors, stimulates JNK, and induces filopodial formation.

NCBI and Uniprot Product Information

• NCBI GI #: 50263042
• NCBI GeneID: 23433
• NCBI Accession #: NP_036381.2
• NCBI GenBank Nucleotide #: NP_036381.2
• UniProt Accession #: P17081
• Molecular Weight: ~ 20kDa
• NCBI Official Full Name: rho-related GTP-binding protein RhoQ
• NCBI Official Synonym Full Names: ras homolog family member Q
• NCBI Official Symbol: RHOQ
• NCBI Official Synonym Symbols: ARHQ; TC10; TC10A; RASL7A; HEL-S-42
• NCBI Protein Information: rho-related GTP-binding protein RhoQ
• UniProt Protein Name: Rho-related GTP-binding protein RhoQ
• Protein Family: Rho-related GTP-binding protein
• UniProt Gene Name: RHOQ
• UniProt Synonym Gene Names: ARHQ; RASL7A; TC10
• UniProt Entry Name: RHOQ_HUMAN

NCBI Description

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The encoded protein is an important signalling protein for sarcomere assembly and has been shown to play a significant role in the exocytosis of the solute carrier family 2, facilitated glucose transporter member 4 and other proteins, possibly acting as the signal that turns on the membrane fusion machinery. Three related pseudogene have been identified on chromosomes 2 and 14. [provided by RefSeq, Aug 2011]

Uniprot Description

RHOQ: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia. Belongs to the small GTPase superfamily. Rho family.

Protein type: G protein, monomeric; G protein; G protein, monomeric, Rho; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: plasma membrane; actin filament; cytosol; lipid raft

Molecular Function: GTPase activity; GTP binding; GBD domain binding; profilin binding

Biological Process: positive regulation of filopodium formation; regulation of cell shape; positive regulation of glucose import; regulation of small GTPase mediated signal transduction; cellular response to insulin stimulus; metabolic process; regulation of actin cytoskeleton organization and biogenesis; small GTPase mediated signal transduction; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; cortical actin cytoskeleton organization and biogenesis