Western Blot (WB)
(Western blot analysis of RhoGDI alpha (pS174) expression in HeLa (A), Jurkat (B) whole cell lysates.)
Rabbit RhoGDI alpha Polyclonal Antibody | anti-ARHGDIA antibody
Anti-RhoGDI alpha (pS174) Phospho Antibody
Western Blot (WB)
(Western blot analysis of RhoGDI alpha (pS174) expression in HeLa (A), Jurkat (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of RhoGDI alpha (pS174) expression in HeLa (A), Jurkat (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of RhoGDI alpha (pS174) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of RhoGDI alpha (pS174) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Uniprot Description
RhoGDI alpha: Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Monomer. Forms a heterodimer with RAC1. Interacts with FER. Belongs to the Rho GDI family.
Protein type: Apoptosis; Cell development/differentiation; G protein regulator, misc.; Cell adhesion; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: cytoskeleton; cytosol
Molecular Function: GTPase activator activity; protein binding; Rho GDP-dissociation inhibitor activity
Biological Process: cell motility; negative regulation of apoptosis; negative regulation of axonogenesis; negative regulation of cell adhesion; nerve growth factor receptor signaling pathway; positive regulation of axonogenesis; positive regulation of GTPase activity; regulation of axonogenesis; regulation of catalytic activity; regulation of small GTPase mediated signal transduction; Rho protein signal transduction; small GTPase mediated signal transduction
Disease: Nephrotic Syndrome, Type 8
Research Articles on ARHGDIA
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Product Notes
The ARHGDIA arhgdia (Catalog #AAA8242027) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RhoGDI alpha (pS174) Phospho Antibody reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's RhoGDI alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the ARHGDIA arhgdia for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RhoGDI alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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