Rabbit anti-Human RhoA Polyclonal Antibody | anti-RHOA antibody

Anti-RhoA Antibody, Rabbit Polyclonal

Cat. Num. AAA8106443

• Gene Names: RHOA; ARHA; ARH12; RHO12; RHOH12
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: RhoA; Polyclonal Antibody; Anti-RhoA Antibody; Rabbit Polyclonal; RhoA Antibody; Rabbit PAb; Antigen Affinity Purified; ras homolog family member A; Anti-ARH12 Antibody; Anti-ARHA Antibody; Anti-RHO12 Antibody; Anti-RHOH12 Antibody; anti-RHOA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human RhoA
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-RHOA antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human RhoA protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human RhoA (rh RhoA). RhoA specific IgG was purified by Human RhoA affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(RHOA was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-RHOA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-RHOA rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 21 kDaObserved band size: 21 kDa)

Western Blot (WB)

(Anti-RHOA rabbit polyclonal antibody at 1:500 dilutionLane A: HL60 Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: SKBR3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:18 kDa)

Western Blot (WB)

(Anti-RHOA rabbit polyclonal antibody at 1:500 dilution Lane A: RHOA konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:21 kDa Observed band size:21 kDa)

Related Product Information for anti-RHOA antibody

Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein that belongs to the small GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA/ARH12 is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division. RHOA/ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila)). RHOA/ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA/ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA/ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP.

Product Categories/Family for anti-RHOA antibody

EGFR Signaling Pathway

References

Kiss C, et al., 1997, Cytogenet. Cell Genet. 79 (3-4): 228-30. Anastasiadis, P.Z. et al., 2000, Nat Cell Biol. 2 (9):637-44. Yiu G, et al., 2006, Nat. Rev. Neurosci. 7 (8): 617-27. Wang, HR. et al., 2006, Methods Enzymol. 406 :437-47. Heo, J. et al., 2006, Biochemistry. 45 (48):14481-9.

NCBI and Uniprot Product Information

• NCBI GI #: 47606458
• NCBI GeneID: 387
• UniProt Accession #: P61586; P06749; Q53HM4; Q5U024; Q9UDJ0; Q9UEJ4
• Molecular Weight: 193
• NCBI Official Full Name: Transforming protein RhoA
• NCBI Official Synonym Full Names: ras homolog family member A
• NCBI Official Symbol: RHOA
• NCBI Official Synonym Symbols: ARHA; ARH12; RHO12; RHOH12
• NCBI Protein Information: transforming protein RhoA; h12; oncogene RHO H12; rho cDNA clone 12; Aplysia ras-related homolog 12; small GTP binding protein RhoA; ras homolog gene family, member A
• UniProt Protein Name: Transforming protein RhoA
• Protein Family: GTP-binding protein
• UniProt Gene Name: RHOA
• UniProt Synonym Gene Names: ARH12; ARHA; RHO12; h12
• UniProt Entry Name: RHOA_HUMAN

Uniprot Description

RHOA: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.

Protein type: G protein, monomeric, Rho; G protein, monomeric; Motility/polarity/chemotaxis; Oncoprotein; G protein

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: apical junction complex; focal adhesion; cytoskeleton; lamellipodium; plasma membrane; cell cortex; midbody; cytosol; cell junction; cleavage furrow

Molecular Function: GTPase activity; protein binding; myosin binding; GTP binding

Biological Process: axon guidance; nerve growth factor receptor signaling pathway; viral reproduction; metabolic process; negative chemotaxis; positive regulation of NF-kappaB import into nucleus; regulation of axonogenesis; regulation of cell migration; Rho protein signal transduction; regulation of osteoblast proliferation; substantia nigra development; transforming growth factor beta receptor signaling pathway; small GTPase mediated signal transduction; positive regulation of stress fiber formation; ephrin receptor signaling pathway; negative regulation of axonogenesis; positive regulation of cytokinesis; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; phosphoinositide-mediated signaling; apical junction assembly; regulation of small GTPase mediated signal transduction; positive regulation of axonogenesis; actin cytoskeleton organization and biogenesis; positive regulation of neuron differentiation; vascular endothelial growth factor receptor signaling pathway; blood coagulation