Rabbit RHBDF2 Polyclonal Antibody | anti-RHBDF2 antibody
RHBDF2, NT (RHBDF2, IRHOM2, RHBDL5, RHBDL6, Inactive rhomboid protein 2, Rhomboid 5 homolog 2, Rhomboid family member 2, Rhomboid veinlet-like protein 5, Rhomboid veinlet-like protein 6)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
IHC (FFPE): 1:25
Requires HIER using sodium citrate buffer pH6.0 prior to staining.
Optimal dilutions to be determined by the researcher.
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of RHBDF2 in formalin fixed paraffin embedded human duodenum tissue using MBS646556 (1:25). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at RT;antigen retrieval was by heat mediation with a citrate buffer (pH6).Samples were incubated with MBS646556 (1:25) for 1 hour at 37°C. Aundiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of RHBDF2 in formalin fixed paraffin embedded in human kidney tissue using MBS646556 (1:25). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at RT;antigen retrieval was by heat mediation with a citrate buffer (pH6).Samples were incubated with MBS646556 (1:25) for 1 hour at 37°C. Aundiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Western Blot (WB)
(Western Blot analysis of mouse lung tissue lysate (20ug/lane) using MBS646556 (1:2000).Secondary Antibody: IgG (HRP) Goat anti-rabbit (1:10,000).)
Western Blot (WB)
(Western Blot analysis of HACAT whole cell lysate (20ug/lane) using MBS646556 (1:2000).Secondary Antibody: IgG (HRP) Goat anti-rabbit (1:10,000).)
Western Blot (WB)
(Western Blot analysis of whole cell lysates (20ug/lane) using 041020(1:1000).Lane 1: RPMI 8226 whole cell lysateLane 2: A549 whole cell lysateLane 3: A431 whole cell lysateLane 4: HT-1080 whole cell lysateLane 5: MCF-7 whole cell lysateSecondary Antibody: IgG (HRP) Goat anti-rabbit (1:10,000).Blocking: 5% NFDM/TBST)
NCBI and Uniprot Product Information
Uniprot Description
Function: Rhomboid protease-like protein which has no protease activity but regulates the secretion of several ligands of the epidermal growth factor receptor. Indirectly activates the epidermal growth factor receptor signaling pathway and may thereby regulate sleep, cell survival, proliferation and migration
By similarity.
Subunit structure: Interacts with EGF
By similarity.
Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein
By similarity. Cell membrane Ref.7.
Tissue specificity: Found in the epidermis and esophageal epithelium. Ref.7
Involvement in disease: Tylosis with esophageal cancer (TOC) [MIM:148500]: An autosomal dominant syndrome characterized by diffuse palmoplantar keratoderma, oral leukokeratosis, and a high lifetime risk of esophageal cancer.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.7
Sequence similarities: Belongs to the peptidase S54 family.
Sequence caution: The sequence AAH35829.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence BAB15310.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Research Articles on RHBDF2
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Product Notes
The RHBDF2 rhbdf2 (Catalog #AAA646556) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RHBDF2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1000 - 1:2000 IHC (FFPE): 1:25 Requires HIER using sodium citrate buffer pH6.0 prior to staining. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the RHBDF2 rhbdf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RHBDF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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