Canine L-phenylalanine ELISA Kit | LPA elisa kit
Canine L-phenylalanine ELISA Kit
Reactivity
Canine
Synonyms
L-phenylalanine; Canine L-phenylalanine ELISA Kit; LPA elisa kit
Reactivity
Canine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LPA elisa kit
Intended Uses: This LPA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine LPA. This ELISA kit for research use only!
Principle of the Assay: LPA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LPA antibody and an LPA-HRP conjugate. The assay sample and buffer are incubated together with LPA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LPA concentration since LPA from samples and LPA-HRP conjugate compete for the anti-LPA antibody binding site. Since the number of sites is limited, as more sites are occupied by LPA from the sample, fewer sites are left to bind LPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LPA concentration in each sample is interpolated from this standard curve.
Principle of the Assay: LPA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LPA antibody and an LPA-HRP conjugate. The assay sample and buffer are incubated together with LPA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LPA concentration since LPA from samples and LPA-HRP conjugate compete for the anti-LPA antibody binding site. Since the number of sites is limited, as more sites are occupied by LPA from the sample, fewer sites are left to bind LPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LPA concentration in each sample is interpolated from this standard curve.
