Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Knockout Validation (Knockout Validation: Lane 1: Wild-type A549 cell lysate; Lane 2: RHD knockout A549 cell lysate; Predicted MW: 34,35,42,43,45,54kd Observed MW: 58kd Primary Ab: 1ug/ml Rabbit Anti-Human RHD Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Human Rh Blood Group, D Antigen (RHD) Polyclonal Antibody | anti-RHD antibody

HRP-Linked Polyclonal Antibody to Rh Blood Group, D Antigen (RHD)

Gene Names
RHD; RH; Rh4; RH30; RhII; RhPI; DIIIc; RHCED; RHDel; RHPII; RhDCw; CD240D; RHXIII; RHDVA(TT); RhK562-II
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Rh Blood Group; D Antigen (RHD); Polyclonal Antibody; HRP-Linked Polyclonal Antibody to Rh Blood Group; CD240D; Rh30a; Rh4; RhPI; RhII; DIIIc; Rhesus D antigen; Rh polypeptide 2; anti-RHD antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a Rabbit polyclonal antibody raised against RHD. It has been selected for its ability to recognize RHD in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid
Concentration
500ug/ml (varies by lot)
Sequence Length
321
Applicable Applications for anti-RHD antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Conjugation
HRP
Cross Reactivity
Human
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Knockout Validation

(Knockout Validation: Lane 1: Wild-type A549 cell lysate; Lane 2: RHD knockout A549 cell lysate; Predicted MW: 34,35,42,43,45,54kd Observed MW: 58kd Primary Ab: 1ug/ml Rabbit Anti-Human RHD Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Knockout Validation (Knockout Validation: Lane 1: Wild-type A549 cell lysate; Lane 2: RHD knockout A549 cell lysate; Predicted MW: 34,35,42,43,45,54kd Observed MW: 58kd Primary Ab: 1ug/ml Rabbit Anti-Human RHD Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
blood group Rh(D) polypeptide isoform 2
NCBI Official Synonym Full Names
Rh blood group D antigen
NCBI Official Symbol
RHD
NCBI Official Synonym Symbols
RH; Rh4; RH30; RhII; RhPI; DIIIc; RHCED; RHDel; RHPII; RhDCw; CD240D; RHXIII; RHDVA(TT); RhK562-II
NCBI Protein Information
blood group Rh(D) polypeptide
UniProt Protein Name
Blood group Rh(D) polypeptide
UniProt Gene Name
RHD
UniProt Synonym Gene Names
RhPII

NCBI Description

The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene, which encodes the RhD protein, and a second gene that encodes both the RhC and RhE antigens on a single polypeptide. The two genes, and a third unrelated gene, are found in a cluster on chromosome 1. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane.

Research Articles on RHD

Similar Products

Product Notes

The RHD rhd (Catalog #AAA2110869) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HRP-Linked Polyclonal Antibody to Rh Blood Group, D Antigen (RHD) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Rh Blood Group, D Antigen (RHD) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the RHD rhd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rh Blood Group, D Antigen (RHD), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.