Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Goat RGS18 Polyclonal Antibody | anti-RGS18 antibody

Goat anti-RGS18 Antibody

Gene Names
RGS18; RGS13
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
RGS18; Polyclonal Antibody; Goat anti-RGS18 Antibody; RGS13; regulator of G-protein signalling 18; regulator of G-protein signaling 13; RP11-142L4.1; regulator of G-protein signalling 13; RGS18 antibody; RGS13 antibody; regulator of G-protein signalling 18 antibody; regulator of G-protein signaling 13 antibody; RP11-142L4.1 antibody; regulator of G-protein signalling 13 antibody; anti-RGS18 antibody
Ordering
 
When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures.
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
CNEFQDVQSDVAIWL
Sequence Length
235
Applicable Applications for anti-RGS18 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 28kDa band observed in Human Peripheral Blood Mononucleocytes (lymphocytes) lysates (calculated MW of 27.6kDa according to NP_570138.1). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence CNEFQDVQSDVAIWL, from the C Terminus of the protein sequence according to NP_570138.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Human Peripheral Blood Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,582 Da
NCBI Official Full Name
regulator of G-protein signaling 18
NCBI Official Synonym Full Names
regulator of G-protein signaling 18
NCBI Official Symbol
RGS18
NCBI Official Synonym Symbols
RGS13
NCBI Protein Information
regulator of G-protein signaling 18
UniProt Protein Name
Regulator of G-protein signaling 18
UniProt Gene Name
RGS18
UniProt Synonym Gene Names
RGS13; RGS18
UniProt Entry Name
RGS18_HUMAN

NCBI Description

This gene encodes a member of the regulator of G-protein signaling family. This protein is contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signaling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

RGS18: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i) alpha-1, G(i) alpha- 2, G(i) alpha-3 and G(q) alpha.

Protein type: GAPs; GAPs, RGS

Chromosomal Location of Human Ortholog: 1q31.2

Cellular Component: cytoplasm; plasma membrane

Molecular Function: GTPase activator activity

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of signal transduction; positive regulation of GTPase activity; regulation of G-protein coupled receptor protein signaling pathway

Research Articles on RGS18

Similar Products

Product Notes

The RGS18 rgs18 (Catalog #AAA420737) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-RGS18 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's RGS18 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 28kDa band observed in Human Peripheral Blood Mononucleocytes (lymphocytes) lysates (calculated MW of 27.6kDa according to NP_570138.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the RGS18 rgs18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CNEFQDVQSD VAIWL. It is sometimes possible for the material contained within the vial of "RGS18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.
Looking for a specific manual?
Request a Manual