Rabbit anti-Human, Mouse RGS14 Polyclonal Antibody | anti-RGS14 antibody

RGS14 Polyclonal Antibody

Cat. Num. AAA3009359

• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RGS14; Polyclonal Antibody; RGS14 Polyclonal Antibody; Regulator of G-protein signaling 14; anti-RGS14 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RGS14 polyclonal antibody detects endogenous levels of RGS14 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 566

• Applicable Applications for anti-RGS14 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant protein of human RGS14.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cells, using RGS14 antibody.)

Related Product Information for anti-RGS14 antibody

RGS domains bind directly to activated Ga subunits and act as GTPase-activating proteins (GAPs) to attenuate and/or modulate hormone and neurotransmitter receptor-initiated signaling by both Ga-GTP and Gbg. RGS proteins shorten the lifetime of the activated G protein. Western blot analysis shows strong expression of RGS14 as a primarily cytosolic protein restricted to brain and spleen. It is suggested that RGS14 may constitute a bridging molecule that allows cross-regulation of signaling pathways downstream from G protein-coupled receptors.

NCBI and Uniprot Product Information

• NCBI GI #: 21361304
• NCBI GeneID: 10636
• NCBI Accession #: NP_006471.2
• NCBI GenBank Nucleotide #: NP_006471.2
• UniProt Accession #: O43566
• Molecular Weight: ~ 54kDa
• NCBI Official Full Name: regulator of G-protein signaling 14 isoform 1
• NCBI Official Synonym Full Names: regulator of G protein signaling 14
• NCBI Official Symbol: RGS14
• NCBI Protein Information: regulator of G-protein signaling 14
• UniProt Protein Name: Regulator of G-protein signaling 14
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS14
• UniProt Synonym Gene Names: RGS14
• UniProt Entry Name: RGS14_HUMAN

NCBI Description

This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

RGS14: Acts as a regulator of G protein signaling (RGS). Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI). Confers GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not G(o) alpha subunit GNAO1 and G(i) alpha subunit GNAI2. Confers GAP activity on G(o) alpha subunit GNAI0 and G(i) alpha subunits GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS1 and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division. Probably required for the nerve growth factor (NGF)-mediated neurite outgrowth. May be involved in visual memory processing capacity and hippocampal-based learning and memory. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; GAPs, RGS

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: spindle pole; centrosome; microtubule; PML body; dendrite; postsynaptic density; dendritic spine; nuclear body; postsynaptic membrane; cytoplasm; plasma membrane; spindle; nucleus; cell junction

Molecular Function: receptor signaling complex scaffold activity; microtubule binding; GDP-dissociation inhibitor activity; receptor signaling protein activity; GTPase activating protein binding; G-protein alpha-subunit binding; protein kinase binding; GTPase activator activity

Biological Process: regulation of transcription in response to stress; negative regulation of MAP kinase activity; mitosis; zygote asymmetric cell division; positive regulation of neurogenesis; negative regulation of synaptic plasticity; platelet-derived growth factor receptor signaling pathway; nucleocytoplasmic transport; learning; spindle organization and biogenesis; chromosome segregation; long-term memory; cell division; visual learning; response to oxidative stress; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity