Rabbit anti-Human RFXANK Polyclonal Antibody | anti-RFXANK antibody

RFXANK, ID (RFXANK, ANKRA1, RFXB, DNA-binding protein RFXANK, Ankyrin repeat family A protein 1, Regulatory factor X subunit B, Regulatory factor X-associated ankyrin-containing protein) (MaxLight 405)

Cat. Num. AAA6341570

• Gene Names: RFXANK; BLS; RFX-B; ANKRA1; F14150_1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: RFXANK; Polyclonal Antibody; ID (RFXANK; ANKRA1; RFXB; DNA-binding protein RFXANK; Ankyrin repeat family A protein 1; Regulatory factor X subunit B; Regulatory factor X-associated ankyrin-containing protein) (MaxLight 405); Regulatory factor X-associated ankyrin-containing protein; anti-RFXANK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RFXANK.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-RFXANK antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 94-123 in the Central region of human RFXANK.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in WiDr cell line lysates (35ug/lane) usingMBS6012237. This demonstrates MBS6012237 detected the RFXANK protein (arrow).)

Related Product Information for anti-RFXANK antibody

Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Two transcript variants encoding different isoforms have been described for this gene, with only one isoform showing activation activity.

Product Categories/Family for anti-RFXANK antibody

Antibodies; Binding Proteins

References

1. Garvie, C.W., et al. Biochim. Biophys. Acta 1779(12):797-804(2008). 2. Ewing, R.M., et al. Mol. Syst. Biol. 3:89 (2007). 3. Krawczyk, M., et al. Mol. Cell. Biol. 25(19):8607-8618(2005). 4. Wang, A.H., et al. J. Biol. Chem. 280(32):29117-29127(2005). 5. Grimwood, J., et al. Nature 428(6982):529-535(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 19924156
• NCBI GeneID: 8625
• NCBI Accession #: NP_604389.1; NP_003712.1
• NCBI GenBank Nucleotide #: NM_134440.1
• UniProt Accession #: O14593
• NCBI Official Full Name: DNA-binding protein RFXANK isoform b
• NCBI Official Synonym Full Names: regulatory factor X associated ankyrin containing protein
• NCBI Official Symbol: RFXANK
• NCBI Official Synonym Symbols: BLS; RFX-B; ANKRA1; F14150_1
• NCBI Protein Information: DNA-binding protein RFXANK
• UniProt Protein Name: DNA-binding protein RFXANK
• Protein Family: DNA-binding protein
• UniProt Gene Name: RFXANK
• UniProt Synonym Gene Names: ANKRA1; RFXB; RFX-B

NCBI Description

Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator/CIITA. May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters (PubMed:9806546, PubMed:10072068, PubMed:10725724). May also potentiate the activation of RAF1 ().