Aquaporin 0 Polyclonal Antibody | anti-MIP antibody

Anti-Aquaporin 0 Antibody

Cat. Num. AAA177668

• Gene Names: MIP; AQP0; LIM1; MP26; MIP26; CTRCT15
• Reactivity: Rat; Predicted to work with: Human
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: Aquaporin 0; Polyclonal Antibody; Anti-Aquaporin 0 Antibody; Lens fiber major intrinsic protein; AQP 0; AQP0; Aquaporin; Aquaporin0; LIM 1; LIM1; Major intrinsic protein 26kD; Major intrinsic protein of lens fiber; MIP 26; MIP; MIP26; MP 26; MP26; PALM; major intrinsic protein of lens fiber; anti-MIP antibody

• Reactivity: Rat; Predicted to work with: Human
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 263

• Applicable Applications for anti-MIP antibody: Western Blot (WB)
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 0 (232-263aa ERLSVLKGAKPDVSNGQPEVTGEPVELNTQAL), different from the related mouse and rat sequences by six amino acids.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- Aquaporin 0 Picoband antibody, MBS177668, Western blottingAll lanes: Anti Aquaporin 0 (MBS177668) at 0.5ug/mlWB: Rat Eye Ball Tissue Lysate at 50ugPredicted bind size: 28KDObserved bind size: 28KD )

Related Product Information for anti-MIP antibody

Description: Rabbit IgG polyclonal antibody for Lens fiber major intrinsic protein(MIP) detection. Tested with WB in Human;Rat.

Background: Lens fiber major intrinsic protein, also called MIP26 or MP26, is a protein that in humans is encoded by the MIP gene. MIP is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. Using 2-color fluorescence in situ hybridization on high-resolution R-banded chromosomes and human genomic DNA clones for MIP as probes, this gene was found that located in close proximity in region 12q13. MIP plays a crucial role in the development of a transparent eye lens. This gene may be responsible for regulating the osmolarity of the lens and interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core.

References

1. Berry, V., Francis, P., Kaushal, S., Moore, A., Bhattacharya, S. Missense mutations in MIP underlie autosomal dominant 'polymorphic' and lamellar cataracts linked to 12q. Nature Genet. 25: 15-17, 2000. 2. Francis, P., Chung, J.-J., Yasui, M., Berry, V., Moore, A., Wyatt, M. K., Wistow, G., Bhattacharya, S. S., Agre, P. Functional impairment of lens aquaporin in two families with dominantly inherited cataracts. Hum. Molec. Genet. 9: 2329-2334, 2000. 3. Pisano MM, Chepelinsky AB (Mar 1992). "Genomic cloning, complete nucleotide sequence, and structure of the human gene encoding the major intrinsic protein (MIP) of the lens". Genomics 11 (4): 981-90.

NCBI and Uniprot Product Information

• NCBI GI #: 6912506
• NCBI GeneID: 4284
• NCBI Accession #: NP_036196.1
• NCBI GenBank Nucleotide #: NM_012064.3
• UniProt Accession #: P30301; Q17R41
• Molecular Weight: 28,122 Da
• NCBI Official Full Name: lens fiber major intrinsic protein
• NCBI Official Synonym Full Names: major intrinsic protein of lens fiber
• NCBI Official Symbol: MIP
• NCBI Official Synonym Symbols: AQP0; LIM1; MP26; MIP26; CTRCT15
• NCBI Protein Information: lens fiber major intrinsic protein
• UniProt Protein Name: Lens fiber major intrinsic protein
• Protein Family: Allatostatins
• UniProt Gene Name: MIP
• UniProt Synonym Gene Names: AQP0; MP26
• UniProt Entry Name: MIP_HUMAN

NCBI Description

Major intrinsic protein is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. The function of the fiber cell membrane protein encoded by this gene is undetermined, yet this protein is speculated to play a role in intracellular communication. The MIP protein is expressed in the ocular lens and is required for correct lens function. This gene has been mapped among aquaporins AQP2, AQP5, and AQP6, in a potential gene cluster at 12q13. [provided by RefSeq, Jul 2008]

Uniprot Description

AQP0: major intrinsic protein of lens fiber. A member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. May be responsible for regulating the osmolarity of the lens. Defects in MIP are a cause of autosomal recessive congenital cataract.

Protein type: Membrane protein, integral; Transporter, aquaporin family; Channel, misc.; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: apical plasma membrane; endoplasmic reticulum; gap junction; integral to plasma membrane; intracellular canaliculus; plasma membrane

Molecular Function: calmodulin binding; gap junction channel activity; glycerol channel activity; structural constituent of eye lens; transporter activity; water channel activity

Biological Process: canalicular bile acid transport; cellular water homeostasis; glycerol transport; lens development in camera-type eye; positive regulation of cell adhesion; protein homotetramerization; response to stimulus; visual perception; water transport

Disease: Cataract 15, Multiple Types