Rabbit RFX2 Polyclonal Antibody | anti-RFX2 antibody

RFX2 Antibody

Cat. Num. AAA9631071

• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: RFX2; Polyclonal Antibody; RFX2 Antibody; DNA-binding protein RFX2; HLA class II regulatory factor RFX2; regulatory factor X 2 (influences HLA class II expression); Regulatory factor X 2; regulatory factor X2; RFX2_HUMAN; trans acting regulatory factor 2; anti-RFX2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: RFX2 Antibody detects endogenous levels of RFX2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RFX2 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human RFX2.
• Conjugation: Unconjugated
• Similarity: Belongs to the RFX family.
• Subunit Structure: Homodimer; probably only forms homodimers in testis (By similarity). Heterodimer; heterodimerizes with RFX1 and RFX3.
• Function: Transcription factor that acts as a key regulator of spermatogenesis. Acts by regulating expression of genes required for the haploid phase during spermiogenesis, such as genes required for cilium assembly and function (By similarity). Recognizes and binds the X-box, a regulatory motif with DNA sequence 5'-GTNRCC(0-3N)RGYAAC-3' present on promoters. Probably activates transcription of the testis-specific histone gene HIST1H1T (By similarity).
• Note: Mainly expressed in the nucleus and at lower level in cytoplasm.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-RFX2 antibody

Nucleus; Cytoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 19743881
• NCBI GeneID: 5990
• NCBI Accession #: NP_000626.2
• NCBI GenBank Nucleotide #: NM_000635.3
• UniProt Accession #: P48378; Q6IQ44; Q8SNA2; A8K581; B3KNC4
• Molecular Weight: 79,987 Da
• NCBI Official Full Name: DNA-binding protein RFX2 isoform a
• NCBI Official Synonym Full Names: regulatory factor X, 2 (influences HLA class II expression)
• NCBI Official Symbol: RFX2
• NCBI Protein Information: DNA-binding protein RFX2; regulatory factor X 2; DNA binding protein RFX2; trans-acting regulatory factor 2; HLA class II regulatory factor RFX2
• UniProt Protein Name: DNA-binding protein RFX2
• Protein Family: RFX-like DNA-binding protein
• UniProt Gene Name: RFX2
• UniProt Entry Name: RFX2_HUMAN

NCBI Description

This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. This protein can bind to cis elements in the promoter of the IL-5 receptor alpha gene. Two transcript variants encoding different isoforms have been described for this gene, and both variants utilize alternative polyadenylation sites. [provided by RefSeq, Jul 2008]

Uniprot Description

RFX2: a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by structurally related to regulatory factors X1, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. This protein can bind to cis elements in the promoter of the IL-5 receptor alpha gene. Two transcript variants encoding different isoforms have been described for this gene, and both variants utilize alternative polyadenylation sites. [provided by RefSeq, Jul 2008]

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleus

Molecular Function: DNA binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent