Western Blot (WB)
(RFT1 Antibody (C-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the RFT1 antibody detected the RFT1 protein (arrow).)
Rabbit anti-Human RFT1 Polyclonal Antibody | anti-RFT1 antibody
RFT1, CT (RFT1, Protein RFT1 homolog) (PE)
Gene Names
RFT1; CDG1N
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
RFT1; Polyclonal Antibody; CT (RFT1; Protein RFT1 homolog) (PE); Protein RFT1 homolog; requiring fifty three 1 homolog (S. cerevisiae); anti-RFT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-RFT1 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
RFT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-549 amino acids from the C-terminal region of human RFT1.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(RFT1 Antibody (C-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the RFT1 antibody detected the RFT1 protein (arrow).)
Western Blot (WB)
(RFT1 Antibody (C-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the RFT1 antibody detected the RFT1 protein (arrow).)
Immunohistochemistry (IHC)
(RFT1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RFT1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(RFT1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RFT1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Product Categories/Family for anti-RFT1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,335 Da
NCBI Official Full Name
protein RFT1 homolog
NCBI Official Synonym Full Names
RFT1 homolog (S. cerevisiae)
NCBI Official Symbol
RFT1
NCBI Official Synonym Symbols
CDG1N
NCBI Protein Information
protein RFT1 homolog; RFT1, requiring fifty three 1 homolog; congenital disorder of glycosylation 1N; putative endoplasmic reticulum multispan transmembrane protein
UniProt Protein Name
Protein RFT1 homolog
Protein Family
UniProt Gene Name
RFT1
UniProt Entry Name
RFT1_HUMAN
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Product Notes
The RFT1 rft1 (Catalog #AAA6341509) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RFT1, CT (RFT1, Protein RFT1 homolog) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RFT1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-100 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RFT1 rft1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RFT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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