Rabbit anti-Human RFC5 Polyclonal Antibody | anti-RFC5 antibody

RFC5, ID (R185) (Replication Factor C Subunit 5, Activator 1 Subunit 5, Replication Factor C 36kD Subunit, RF-C 36kD Subunit, RFC36, Activator 1 36kD Subunit, A1 36kD Subunit, RFC5) (MaxLight 650)

Cat. Num. AAA6497462

• Gene Names: RFC5; RFC36
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: RFC5; Polyclonal Antibody; ID (R185) (Replication Factor C Subunit 5; Activator 1 Subunit 5; Replication Factor C 36kD Subunit; RF-C 36kD Subunit; RFC36; Activator 1 36kD Subunit; A1 36kD Subunit; RFC5) (MaxLight 650); anti-RFC5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RFC5.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-RFC5 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human RFC5 (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-RFC5 Antibody (Center R185) in Hela cell line lysates (35ug/lane). RFC5 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of anti-RFC5 Antibody (Center R185) in rat PASMC cell line lysates (35ug/lane). RFC5 (arrow) was detected using the purified Pab (Provided by Hirotaka Ata).)

Western Blot (WB)

(Western blot analysis of RFC5 (arrow) using rabbit polyclonal RFC5 Antibody (Center R185). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RFC5 gene.)

Flow Cytometry (FC/FACS)

(RFC5 Antibody (Center R185) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-RFC5 antibody

Antibodies; Transcription Factors

References

1. Stelzl, U., Cell 122(6), 957-968 (2005). 2. Andersen, J.S., Nature 433(7021), 77-83 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 194306571
• NCBI GeneID: 5985
• NCBI Accession #: NP_031396
• NCBI GenBank Nucleotide #: NM_007370
• UniProt Accession #: P40937
• Molecular Weight: 38kDa
• NCBI Official Full Name: replication factor C subunit 5 isoform 2
• NCBI Official Synonym Full Names: replication factor C subunit 5
• NCBI Official Symbol: RFC5
• NCBI Official Synonym Symbols: RFC36
• NCBI Protein Information: replication factor C subunit 5
• UniProt Protein Name: Replication factor C subunit 5
• Protein Family: Replication factor
• UniProt Gene Name: RFC5
• UniProt Entry Name: RFC5_HUMAN

NCBI Description

This gene encodes the smallest subunit of the replication factor C complex, which consists of five distinct subunits (140, 40, 38, 37, and 36 kDa) and is required for DNA replication. This subunit interacts with the C-terminal region of proliferating cell nuclear antigen and is required to open and load proliferating cell nuclear antigen onto DNA during S phase. It is a member of the AAA+ (ATPases associated with various cellular activities) ATPase family and forms a core complex with the 38 and 40 kDa subunits that possesses DNA-dependent ATPase activity. A related pseudogene has been identified on chromosome 9. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]