Western Blot (WB)
(Anti-RFC1 antibody, MBS175156, Western blottingAll lanes: Anti RFC1 (MBS175156) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: SMMC Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: CEM Whole Cell Lysate at 40ugLane 5: 293T Whole Cell Lysate at 40ugPredicted bind size: 128KDObserved bind size: 150KD)
Rabbit anti-Human RFC1 Polyclonal Antibody | anti-RFC1 antibody
Anti-RFC1 antibody
Western Blot (WB)
(Anti-RFC1 antibody, MBS175156, Western blottingAll lanes: Anti RFC1 (MBS175156) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: SMMC Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: CEM Whole Cell Lysate at 40ugLane 5: 293T Whole Cell Lysate at 40ugPredicted bind size: 128KDObserved bind size: 150KD)
Western Blot (WB)
(Anti-RFC1 antibody, MBS175156, Western blottingAll lanes: Anti RFC1 (MBS175156) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: SMMC Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: CEM Whole Cell Lysate at 40ugLane 5: 293T Whole Cell Lysate at 40ugPredicted bind size: 128KDObserved bind size: 150KD)
Immunohistochemistry (IHC)
(Anti-RFC1 antibody, MBS175156, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-RFC1 antibody, MBS175156, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Background: RFC(Replication Factor C), also called activator-1, is a multimeric primer-recognition protein consisting of 5 distinct subunits of 145, 40, 38, 37, and 36.5 kD. Human RFC was purified from extracts of HeLa cells as a host factor essential for the in vitro replication of simian virus 40(SV40) DNA. RFC, in the presence of ATP, assembles proliferating-cell nuclear antigen and DNA polymerase-delta or polymerase-epsilon on primed DNA templates. Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]
Uniprot Description
RFC1: a DNA binding protein that may play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and RFC1. The RFC1 subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Two alternatively spliced isoforms have been described.
Protein type: DNA replication
Chromosomal Location of Human Ortholog: 4p14-p13
Cellular Component: nucleoplasm; Golgi apparatus; DNA replication factor C complex; nucleolus; nucleus; cell junction
Molecular Function: protein domain specific binding; protein binding; DNA binding; sequence-specific DNA binding; double-stranded DNA binding; enzyme activator activity; ATP binding; DNA clamp loader activity
Biological Process: positive regulation of catalytic activity; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; telomere maintenance via telomerase; negative regulation of transcription from RNA polymerase II promoter; DNA strand elongation during DNA replication; DNA repair; telomere maintenance via semi-conservative replication; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; DNA-dependent DNA replication; telomere maintenance
Research Articles on RFC1
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Product Notes
The RFC1 rfc1 (Catalog #AAA175156) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RFC1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RFC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the RFC1 rfc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RFC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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