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Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells, using RFA2 Antibody. The picture on the right is blocked with the synthesized peptide.)

Rabbit anti-Human, Mouse RFA2 Polyclonal Antibody | anti-RFA2 antibody

RFA2 Antibody

Gene Names
RPA2; REPA2; RPA32; RP-A p32; RP-A p34
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
RFA2; Polyclonal Antibody; RFA2 Antibody; anti-RFA2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
RFA2 Antibody detects endogenous levels of total RFA2 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
358
Applicable Applications for anti-RFA2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:1000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human RFA2.
Immunogen Range
Oct-59
Preparation and Storage
Stable at -20 degree C for at least 1 year.

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells, using RFA2 Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells, using RFA2 Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using RFA2 Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using RFA2 Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from K562 cells, using RFA2 Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB) (Western blot analysis of lysates from K562 cells, using RFA2 Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29 kDa
NCBI Official Full Name
replication protein A 32 kDa subunit isoform 2
NCBI Official Synonym Full Names
replication protein A2
NCBI Official Symbol
RPA2
NCBI Official Synonym Symbols
REPA2; RPA32; RP-A p32; RP-A p34
NCBI Protein Information
replication protein A 32 kDa subunit
UniProt Protein Name
Replication protein A 32 kDa subunit
UniProt Gene Name
RPA2
UniProt Synonym Gene Names
REPA2; RPA32; RPA34; RP-A p32; RF-A protein 2; RP-A p34

NCBI Description

This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017]

Uniprot Description

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

Research Articles on RFA2

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Product Notes

The RFA2 rpa2 (Catalog #AAA9503903) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RFA2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RFA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000. Researchers should empirically determine the suitability of the RFA2 rpa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RFA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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