Rabbit anti-Human, Mouse CDH3 / P-Cadherin Polyclonal Antibody | anti-CDH3 antibody

Rabbit Polyclonal to Human CDH3 / P-Cadherin

Cat. Num. AAA248856

• Gene Names: CDH3; CDHP; HJMD; PCAD
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Immunoaffinity Purified
• Synonyms: CDH3 / P-Cadherin; Polyclonal Antibody; Rabbit Polyclonal to Human CDH3 / P-Cadherin; Anti-CDH3 / P-Cadherin Antibody (N-Terminus) IHC-plus; CDH3; Cadherin-3; HJMD; P-cadherin; CDHP; PCAD; Placental cadherin; Human CDH3; P-Cadherin; anti-CDH3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS with 0.09% (w/v) sodium azide
• Sequence Length: 829

• Applicable Applications for anti-CDH3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flo (1:10 - 1:50), IHC-P (10 ug/ml), WB (1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide located between amino acids 114 and 142 of P22223.
• Immunogen: CDH3 / P-Cadherin antibody was raised against synthetic peptide located between amino acids 114 and 142 of P22223.
• Antigen Modification: N-Terminus
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-CDH3 / P-Cadherin antibody IHC staining of human pancreatic duct. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with CDH3 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Flow Cytometry (FC/FACS)

(Flow cytometry of NCI-H292 cells using CDH3 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot of CDH3 Antibody (N-term) in NIH-3T3 cell line lysates (35 ug/lane). CDH3 (arrow) was detected using the purified Pab.)

Related Product Information for anti-CDH3 antibody

CDH3, a classical cadherin, is an integral membrane, calcium-dependent glycoprotein that mediates cell-cell interactions. The protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Alterations in the CDH3 gene have been linked to hypotrichosis with juvenile macular dystrophy (HJMD), and aberrant expression of CDH3 protein has been associated with several types of malignancies, including prostate and breast carcinomas.

Product Categories/Family for anti-CDH3 antibody

Family: Cadherin

NCBI and Uniprot Product Information

• NCBI GI #: 14589891
• NCBI GeneID: 1001
• NCBI Accession #: NP_001784.2
• NCBI GenBank Nucleotide #: NM_001793.4
• UniProt Accession #: P22223; Q05DI6; B2R6F4
• Molecular Weight: 86,552 Da
• NCBI Official Full Name: cadherin-3 preproprotein
• NCBI Official Synonym Full Names: cadherin 3, type 1, P-cadherin (placental)
• NCBI Official Symbol: CDH3
• NCBI Official Synonym Symbols: CDHP; HJMD; PCAD
• NCBI Protein Information: cadherin-3; calcium-dependent adhesion protein, placental
• UniProt Protein Name: Cadherin-3
• UniProt Gene Name: CDH3
• UniProt Synonym Gene Names: CDHP; P-cadherin
• UniProt Entry Name: CADH3_HUMAN

NCBI Description

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy. [provided by RefSeq, Jul 2008]

Uniprot Description

CDH3: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Interacts with CDCP1. Expressed in some normal epithelial tissues and in some carcinoma cell lines. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: cell-cell adherens junction; cytoplasm; integral to membrane; plasma membrane

Molecular Function: calcium ion binding

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; response to drug; intercellular junction assembly and maintenance; wound healing; positive regulation of melanin biosynthetic process; Wnt receptor signaling pathway through beta-catenin; retinal homeostasis; negative regulation of transforming growth factor-beta2 production; hair cycle process; cell-cell adhesion; positive regulation of monophenol monooxygenase activity; negative regulation of catagen; visual perception; keratinization; homophilic cell adhesion; cell adhesion

Disease: Ectodermal Dysplasia, Ectrodactyly, And Macular Dystrophy Syndrome; Hypotrichosis, Congenital, With Juvenile Macular Dystrophy