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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Pig Retinol Binding Protein 7, Cellular (RBP7) Polyclonal Antibody | anti-RBP7 antibody

Polyclonal Antibody to Retinol Binding Protein 7, Cellular (RBP7)

Gene Names
RBP7; CRBP4; CRBPIV
Reactivity
Pig
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Retinol Binding Protein 7; Cellular (RBP7); Polyclonal Antibody; Polyclonal Antibody to Retinol Binding Protein 7; anti-RBP7 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Pig
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against RBP7. It has been selected for its ability to recognize RBP7 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG S-MPADLSGTWN LVSSDNFEGY MLALGIDFAT RKIAKLLKPQ KVIEQNGDSF TIHTNSSLRN YLVKFKVGEE FDEDNKGLDN RKCKSLVTWD NDRLTCVQKG EKKNRGWTHW IEGDELHLEM FCEGQVCKQT FQRA
Sequence Length
134
Applicable Applications for anti-RBP7 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant RBP7 (Met1~Ala134) expressed in E.coli.
Cross Reactivity
Pig
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2083966
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
15,536 Da
NCBI Official Full Name
Retinol binding protein 7, cellular
NCBI Official Synonym Full Names
retinol binding protein 7, cellular
NCBI Official Symbol
RBP7
NCBI Official Synonym Symbols
CRBP4; CRBPIV
NCBI Protein Information
retinoid-binding protein 7; CRABP4; CRABP-IV; retinoid binding protein 7; cellular retinoic acid-binding protein 4; cellular retinoic acid-binding protein IV; putative cellular retinol-binding protein CRBP IV
UniProt Protein Name
Retinoid-binding protein 7
Protein Family
UniProt Gene Name
RBP7
UniProt Synonym Gene Names
CRABP4; CRBP4; CRABP-IV
UniProt Entry Name
RET7_HUMAN

NCBI Description

Due to its chemical instability and low solubility in aqueous solution, vitamin A requires cellular retinol-binding proteins (CRBPs), such as RBP7, for stability, internalization, intercellular transfer, homeostasis, and metabolism.[supplied by OMIM, May 2004]

Uniprot Description

RBP7: Intracellular transport of retinol. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: cytoplasm

Molecular Function: transporter activity; retinol binding; retinal binding

Biological Process: transport

Research Articles on RBP7

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Product Notes

The RBP7 rbp7 (Catalog #AAA2005278) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Retinol Binding Protein 7, Cellular (RBP7) reacts with Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Retinol Binding Protein 7, Cellular (RBP7) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the RBP7 rbp7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG S-MPADLSGT WN LVSSDNFEGY MLALGIDFAT RKIAKLLKPQ KVIEQNGDSF TIHTNSSLRN YLVKFKVGEE FDEDNKGLDN RKCKSLVTWD NDRLTCVQKG EKKNRGWTHW IEGDELHLEM FCEGQVCKQT FQRA. It is sometimes possible for the material contained within the vial of "Retinol Binding Protein 7, Cellular (RBP7), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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