PLAT recombinant protein

PLAT, 311-562aa, Human, His-tag, Baculovirus

Cat. Num. AAA206510

• Gene Names: PLAT; TPA; T-PA
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: PLAT; 311-562aa; Human; His-tag; Baculovirus; Plasminogen activator; tissue type; T-PA; TPA; PLAT recombinant protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 50mM MES (pH 5.0) containing 5mM CaCl2, 1mM DTT, 0.5M NaCl, 30% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPIKGGLFA DIASHPWQAA IFAKHRRSPG ERFLCGGILI SSCWILSAAH CFQERFPPHH LTVILGRTYR VVPGEEEQKF EVEKYIVHKE FDDDTYDNDI ALLQLKSDSS RCAQESSVVR TVCLPPADLQ LPDWTECELS GYGKHEALSP FYSERLKEAH VRLYPSSRCT SQHLLNRTVT DNMLCAGDTR SGGPQANLHD ACQGDSGGPL VCLNDGRMTL VGIISWGLGC GQKDVPGVYT KVTNYLDWIR DNMRPHHHHH H
• Sequence Length: 562

• Applicable Applications for PLAT recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PLAT recombinant protein

PLAT, also known as plasminogen activator, tissue type, is serine protease secreted by many cell types that participate in biological processes, such as tissue restructuring, cell migration, and tumor metastasis. It used to dissolve coronary fibrin clots that are the proximal causes of acute myocardial infarction. It plays a crucial role in regulating blood fibrinolysis, in maintaining the homeostasis of extracellular matrix and in modulating the post-translational activation of growth factors. Recombinant human PLAT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for PLAT recombinant protein

Cardiovascular

References

Orth K., et al. (1992) Proc Natl Acad Sci U S A. 89:7422-7426.; Hu K., et al. (2008) Front Biosci. 13:5174-5186.

NCBI and Uniprot Product Information

• NCBI GI #: 4505861
• NCBI GeneID: 5327
• NCBI Accession #: NP_000921
• NCBI GenBank Nucleotide #: NM_000930.4
• UniProt Accession #: P00750; Q15103; Q503B0; Q6PJA5; Q7Z7N2; Q86YK8; Q9BU99; Q9BZW1; A8K022; B2R8E8
• Molecular Weight: 29.2kDa (261aa) 28-40kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: tissue-type plasminogen activator isoform 1 preproprotein
• NCBI Official Synonym Full Names: plasminogen activator, tissue type
• NCBI Official Symbol: PLAT
• NCBI Official Synonym Symbols: TPA; T-PA
• NCBI Protein Information: tissue-type plasminogen activator
• UniProt Protein Name: Tissue-type plasminogen activator
• Protein Family: Platelet factor
• UniProt Gene Name: PLAT
• UniProt Synonym Gene Names: t-PA; t-plasminogen activator; tPA

NCBI Description

This gene encodes tissue-type plasminogen activator, a secreted serine protease that converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. The encoded preproprotein is proteolytically processed by plasmin or trypsin to generate heavy and light chains. These chains associate via disulfide linkages to form the heterodimeric enzyme. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, while decreased activity leads to hypofibrinolysis, which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration.