Rabbit anti-Human RET Polyclonal Antibody | anti-RET antibody

RET, CT (L1027) (RET, CDHF12, CDHR16, PTC, RET51, Proto-oncogene tyrosine-protein kinase receptor Ret, Cadherin family member 12, Proto-oncogene c-Ret, Soluble RET kinase fragment, Extracellular cell-membrane anchored RET cadherin 120kD fragment) (FITC)

Cat. Num. AAA6341359

• Gene Names: RET; PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: RET; Polyclonal Antibody; CT (L1027) (RET; CDHF12; CDHR16; PTC; RET51; Proto-oncogene tyrosine-protein kinase receptor Ret; Cadherin family member 12; Proto-oncogene c-Ret; Soluble RET kinase fragment; Extracellular cell-membrane anchored RET cadherin 120kD fragment) (FITC); Extracellular cell-membrane anchored RET cadherin 120kD fragment; anti-RET antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RET antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RET antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1020~1050 amino acids from the C-terminal region of human RET.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RET (arrow) using 040965. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RET gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted using 040965, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma)

Related Product Information for anti-RET antibody

RET, a member of the cadherin superfamily, is one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This protein plays a crucial role in neural crest development, and the gene can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations i are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma.

Product Categories/Family for anti-RET antibody

Antibodies; Protein Kinases

References

Da Silva, A.M., et al., J. Clin. Endocrinol. Metab. 88(11):5438-5443 (2003). McWhinney, S.R., et al., J. Clin. Endocrinol. Metab. 88(10):4911-4916 (2003). D'Alessio, A., et al., Endocrinology 144(10):4298-4305 (2003). Soares, P., et al., Oncogene 22(29):4578-4580 (2003). Punales, M.K., et al., J. Clin. Endocrinol. Metab. 88(6):2644-2649 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 10862701
• NCBI GeneID: 5979
• NCBI Accession #: NP_065681.1
• NCBI GenBank Nucleotide #: NM_020630.4
• UniProt Accession #: P07949; Q15250; Q9BTB0; Q9H4A2; A8K6Z2
• Molecular Weight: 124,319 Da
• NCBI Official Full Name: proto-oncogene tyrosine-protein kinase receptor Ret isoform c
• NCBI Official Synonym Full Names: ret proto-oncogene
• NCBI Official Symbol: RET
• NCBI Official Synonym Symbols: PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1
• NCBI Protein Information: proto-oncogene tyrosine-protein kinase receptor Ret; proto-oncogene c-Ret; receptor tyrosine kinase; RET transforming sequence; cadherin family member 12; hydroxyaryl-protein kinase; cadherin-related family member 16; ret proto-oncogene (multiple endocrin
• UniProt Protein Name: Proto-oncogene tyrosine-protein kinase receptor Ret
• Protein Family: Retbindin
• UniProt Gene Name: RET
• UniProt Synonym Gene Names: CDHF12; CDHR16; PTC; RET51
• UniProt Entry Name: RET_HUMAN

NCBI Description

This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed. [provided by RefSeq, Jul 2008]

Uniprot Description

Ret: a proto-oncogenic receptor tyrosine kinase. Receptor for glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin. Part of a multicompetent receptor complex with other membrane-bound ligand-binding GDNF family receptors (aGFRs). Required for development of the kidney and neural crest-derived cell types. Three alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Oncoprotein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Protein kinase, TK; Kinase, protein; TK group; Ret family

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: intracellular membrane-bound organelle; integral to plasma membrane; cytoplasm; plasma membrane; endosome membrane; receptor complex; lipid raft

Molecular Function: protein binding; protein-tyrosine kinase activity; receptor activity; transmembrane receptor protein tyrosine kinase activity; calcium ion binding; ATP binding

Biological Process: response to drug; regulation of cell adhesion; caspase activation; positive regulation of neuron maturation; posterior midgut development; peptidyl-tyrosine phosphorylation; positive regulation of cell size; positive regulation of transcription, DNA-dependent; membrane protein proteolysis; MAPKKK cascade; positive regulation of cell adhesion mediated by integrin; response to pain; regulation of axonogenesis; signal transduction; neuron maturation; protein amino acid phosphorylation; enteric nervous system development; retina development in camera-type eye; ureteric bud development; embryonic epithelial tube formation; neuron adhesion; homophilic cell adhesion; neural crest cell migration; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration

Disease: Multiple Endocrine Neoplasia, Type Iib; Multiple Endocrine Neoplasia, Type Iia; Thyroid Carcinoma, Papillary; Hirschsprung Disease, Susceptibility To, 1; Thyroid Carcinoma, Familial Medullary