Goat anti-Human RENT3A, UPF3 regulator of nonsense transcripts homolog A Polyclonal Antibody | anti-UPF3A antibody

RENT3A, UPF3 regulator of nonsense transcripts homolog A (yeast), UPF3A

Cat. Num. AAA628809

• Gene Names: UPF3A; UPF3; HUPF3A; RENT3A
• Reactivity: Human
• Applications: ELISA
• Purity: Affinity Purified; Purified by peptide affinity chromatography.
• Synonyms: RENT3A; UPF3 regulator of nonsense transcripts homolog A; Polyclonal Antibody; UPF3 regulator of nonsense transcripts homolog A (yeast); UPF3A; Anti -RENT3A; anti-UPF3A antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes RENT3A. Species sequence homology: human and bovine.
• Purity/Purification: Affinity Purified; Purified by peptide affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-UPF3A antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.; Dilution: Peptide ELISA Titer: 1:8000
• Immunogen: Synthetic peptide corresponding to C-KETDKQKKIAEKE, from the internal region of human RENT3A (NP_075387.1; NP_542418.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-UPF3A antibody

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Two splice variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-UPF3A antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 18375525
• NCBI GeneID: 65110
• NCBI Accession #: NP_542418.1
• NCBI GenBank Nucleotide #: NM_080687.2
• UniProt Accession #: Q9H1J1; Q5T8C3; Q5T8C9; Q7Z6N3; Q86YK1; Q9BZI8; A2A366
• Molecular Weight: 54,696 Da
• NCBI Official Full Name: regulator of nonsense transcripts 3A isoform hUpf3pdelta
• NCBI Official Synonym Full Names: UPF3 regulator of nonsense transcripts homolog A (yeast)
• NCBI Official Symbol: UPF3A
• NCBI Official Synonym Symbols: UPF3; HUPF3A; RENT3A
• NCBI Protein Information: regulator of nonsense transcripts 3A; hUpf3; nonsense mRNA reducing factor 3A; up-frameshift suppressor 3 homolog A
• UniProt Protein Name: Regulator of nonsense transcripts 3A
• Protein Family: Regulator of nonsense transcripts
• UniProt Gene Name: UPF3A
• UniProt Synonym Gene Names: RENT3A; UPF3; hUpf3
• UniProt Entry Name: REN3A_HUMAN

NCBI Description

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Two splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

UPF3A: Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2- UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation. Belongs to the RENT3 family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: protein binding; RNA binding; nucleotide binding; nucleocytoplasmic transporter activity

Biological Process: mRNA transport; positive regulation of translation; mRNA catabolic process, nonsense-mediated decay; gene expression; nucleocytoplasmic transport