Western Blot (WB)
(Western blot analysis of Renilla Luciferase expression in (1) Renilla Luciferase transfected 293 cell lysate; (2) HeLa cell lysate; (3) NIH/3T3 cell lysate; (4) C6 cell lysate.)
Rabbit Renilla Luciferase Polyclonal Antibody
Anti-Renilla Luciferase Rabbit pAb
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Affinity Chromatography
Synonyms
Renilla Luciferase; Polyclonal Antibody; Anti-Renilla Luciferase Rabbit pAb; Renilla-type luciferase; Renilla luciferin 2 monooxygenase; anti-Renilla Luciferase antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Renilla Luciferase Antibody detects endogenous levels of total Renilla Luciferase
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
311
Applicable Applications for anti-Renilla Luciferase antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
ICC/IF: 1:50-1:200
Immunogen
A synthesized peptide derived from Renilla Luciferase
Subcellular Location
Nucleus
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot analysis of Renilla Luciferase expression in (1) Renilla Luciferase transfected 293 cell lysate; (2) HeLa cell lysate; (3) NIH/3T3 cell lysate; (4) C6 cell lysate.)
Western Blot (WB)
(Western blot analysis of Renilla Luciferase expression in (1) Renilla Luciferase transfected 293 cell lysate; (2) HeLa cell lysate; (3) NIH/3T3 cell lysate; (4) C6 cell lysate.)
Immunofluorescence (IF)
(Immunofluorescent analysis of 293 cells transfected with Renilla Luciferase, using Renilla Luciferase Antibody.)
Immunofluorescence (IF)
(Immunofluorescent analysis of 293 cells transfected with Renilla Luciferase, using Renilla Luciferase Antibody.)
Related Product Information for anti-Renilla Luciferase antibody
Renilla luciferin + O2 = oxidized Renilla luciferin + CO2 + light. The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly.
NCBI and Uniprot Product Information
Uniprot Description
Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO2 molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.
Similar Products
Product Notes
The Renilla Luciferase (Catalog #AAA4750169) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Renilla Luciferase Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Renilla Luciferase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500-1:2000 ICC/IF: 1:50-1:200. Researchers should empirically determine the suitability of the Renilla Luciferase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Renilla Luciferase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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