Rabbit anti-Mouse Relaxin 3 receptor 1 Polyclonal Antibody | anti-RXFP3 antibody

Anti-Relaxin 3 receptor 1 antibody

Cat. Num. AAA355083

• Gene Names: RXFP3; SALPR; RLN3R1; RXFPR3; GPCR135
• Reactivity: Mouse
• Applications: Western Blot, Immunocytochemistry
• Purity: Immunogen affinity purified
• Synonyms: Relaxin 3 receptor 1; Polyclonal Antibody; Anti-Relaxin 3 receptor 1 antibody; Polyclonal Anti- Relaxin 3 receptor 1; anti-RXFP3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified
• Form/Format: Liquid

• Applicable Applications for anti-RXFP3 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB (1:5000-10,000); ICC (1:100-200)
• Immunogen: A synthetic peptide (conjugated with KLH) corresponding to the region of mouse Relaxin 3 receptor 1.
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
• Preparation and Storage: Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles

Western Blot (WB)

Related Product Information for anti-RXFP3 antibody

Relaxin 3 receptor 1, also called SALPR, is a 469-amino acid protein has the characteristic 7-transmembrane domain structure of a G protein-coupled receptor.[1] Expressed in brain, testis, thymus, and adrenal gland.[2] It did not stimulate cAMP accumulation, but it inhibited forskolin-stimulated cAMP accumulation in GPCR135-expressing cells in a dose-dependent manner.

References

1. Matsumoto, M., Kamohara, M., Sugimoto, T., Hidaka, K., Takasaki, J., Saito, T., Okada, M., Yamaguchi, T., Furuichi, K. The novel G-protein coupled receptor SALPR shares sequence similarity with somatostatin and angiotensin receptors. Gene 248: 183-189, 2000. 2. Liu, C., Eriste, E., Sutton, S., Chen, J., Roland, B., Kuei, C., Farmer, N., Jornvall, H., Sillard, R., Lovenberg, T. W. Identification of relaxin-3/INSL7 as an endogenous ligand for the orphan G-protein-coupled receptor GPCR135. J. Biol. Chem. 278: 50754-50764, 2003.

NCBI and Uniprot Product Information

• NCBI GI #: 7706103
• NCBI GeneID: 51289
• NCBI Accession #: NP_057652.1
• NCBI GenBank Nucleotide #: NM_016568.3
• UniProt Accession #: Q9NSD7; Q14DA5
• Molecular Weight: 51,124 Da
• NCBI Official Full Name: relaxin-3 receptor 1
• NCBI Official Synonym Full Names: relaxin/insulin-like family peptide receptor 3
• NCBI Official Symbol: RXFP3
• NCBI Official Synonym Symbols: SALPR; RLN3R1; RXFPR3; GPCR135
• NCBI Protein Information: relaxin-3 receptor 1; RLN3 receptor 1; relaxin 3 receptor 1; G-protein coupled receptor SALPR; g protein-coupled receptor SALPR; relaxin family peptide receptor 3; G-protein coupled receptor GPCR135; somatostatin and angiotensin-like peptide receptor; somatostatin- and angiotensin-like peptide receptor
• UniProt Protein Name: Relaxin-3 receptor 1
• Protein Family: Relaxin-3 receptor
• UniProt Gene Name: RXFP3
• UniProt Synonym Gene Names: RLN3R1; SALPR; RLN3 receptor 1
• UniProt Entry Name: RL3R1_HUMAN

Uniprot Description

Function: Receptor for RNL3/relaxin-3. Binding of the ligand inhibit cAMP accumulation. Ref.3

Subcellular location: Cell membrane; Multi-pass membrane protein.

Tissue specificity: Expressed predominantly in brain regions. Highest expression in substantia nigra and pituitary, followed by hippocampus, spinal cord, amygdala, caudate nucleus and corpus callosum, quite low level in cerebellum. In peripheral tissues, relatively high levels in adrenal glands, low levels in pancreas, salivary gland, placenta, mammary gland and testis.

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.