Rabbit anti-Human RELA Polyclonal Antibody | anti-RELA antibody

RELA Antibody

Cat. Num. AAA9410226

• Gene Names: RELA; p65; NFKB3
• Reactivity: Human
• Applications: ELISA, Western Blot
• Synonyms: RELA; Polyclonal Antibody; RELA Antibody; v-rel reticuloendotheliosis viral oncogene homolog A (avian); p65; NFKB3; anti-RELA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total RELA protein.
• Form/Format: Supplied at 1mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
• Sequence Length: 2045

• Applicable Applications for anti-RELA antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:2000-1:5000; Western blotting: 1:500-1:2000
• Immunogen Type: Recombinant protein
• Immunogen Description: Full length fusion protein
• Target Name: RELA
• Preparation and Storage: Store at -20 degree C/1 year

Testing Data

(Gel: 10%SDS-PAGELane1: 293T cell lysateLane2: K562 cell lysateLane3: Jurkat cell lysateLysates: 40 ug per lanePrimary antibody: 1/500 dilutionSecondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutionExposure time: 5 seconds)

Related Product Information for anti-RELA antibody

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-RELA antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 15079552
• NCBI GeneID: 5970
• Molecular Weight: 59,910 Da
• NCBI Official Full Name: Homo sapiens v-rel reticuloendotheliosis viral oncogene homolog A (avian), mRNA
• NCBI Official Synonym Full Names: v-rel avian reticuloendotheliosis viral oncogene homolog A
• NCBI Official Symbol: RELA
• NCBI Official Synonym Symbols: p65; NFKB3
• NCBI Protein Information: transcription factor p65
• UniProt Protein Name: Transcription factor p65
• Protein Family: Relaxin
• UniProt Gene Name: RELA
• UniProt Synonym Gene Names: NFKB3
• UniProt Entry Name: TF65_HUMAN

NCBI Description

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.

Protein type: Transcription factor; Nuclear receptor co-regulator; DNA-binding

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; identical protein binding; transcription activator binding; protein N-terminus binding; transcription factor binding; protein kinase binding; NF-kappaB binding; protein binding; DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; protein complex binding; chromatin binding; phosphate binding; transcription factor activity

Biological Process: viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; negative regulation of insulin receptor signaling pathway; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; hair follicle development; toll-like receptor 4 signaling pathway; defense response to virus; response to drug; positive regulation of I-kappaB kinase/NF-kappaB cascade; membrane protein intracellular domain proteolysis; acetaldehyde metabolic process; response to amino acid stimulus; toll-like receptor 2 signaling pathway; organ morphogenesis; positive regulation of chondrocyte differentiation; response to muramyl dipeptide; response to mechanical stimulus; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; response to progesterone stimulus; negative regulation of transcription, DNA-dependent; negative regulation of protein catabolic process; negative regulation of apoptosis; transcription from RNA polymerase II promoter; response to cAMP; response to morphine; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 10 signaling pathway; response to insulin stimulus; response to organic substance; positive regulation of cell proliferation; positive regulation of interferon type I production; inflammatory response; aging; positive regulation of Schwann cell differentiation; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; liver development; response to UV-B; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interleukin-12 biosynthetic process; regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; cellular defense response; response to cobalamin