Western Blot (WB)
(Anti-Rel B antibody, MBS176199, Western blottingLane 1: Rat Testis Tissue LysateLane 2: HELA Cell LysateLane 3: NIH3T3 Cell LysateLane 4: RAJI Cell LysateLane 5: HEPA Cell Lysate )
Rabbit Rel B Polyclonal Antibody | anti-RELB antibody
Anti-Rel B antibody
Gene Names
RELB; IREL; I-REL; REL-B
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
Rel B; Polyclonal Antibody; Anti-Rel B antibody; Transcription factor RelB; v-rel reticuloendotheliosis viral oncogene homolog B; I REL antibody; I-Rel antibody; IREL antibody; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody; relB antibody; RELB_HUMAN antibody; Reticuloendotheliosis viral oncogene homolog B antibody; Transcription factor Rel B antibody; Transcription factor RelB antibody; v rel avian reticuloendotheliosis viral oncogene homolog antibody; v rel avian reticuloendotheliosis viral oncogene homolog B antibody; v rel reticuloendotheliosis viral oncogene homolog B antibody; anti-RELB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
579
Applicable Applications for anti-RELB antibody
Western Blot (WB)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Rel B (393-413aa LPFTYLPRDHDSYGVDKKRKR), identical to the related mouse sequence.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-Rel B antibody, MBS176199, Western blottingLane 1: Rat Testis Tissue LysateLane 2: HELA Cell LysateLane 3: NIH3T3 Cell LysateLane 4: RAJI Cell LysateLane 5: HEPA Cell Lysate )
Western Blot (WB)
(Anti-Rel B antibody, MBS176199, Western blottingLane 1: Rat Testis Tissue LysateLane 2: HELA Cell LysateLane 3: NIH3T3 Cell LysateLane 4: RAJI Cell LysateLane 5: HEPA Cell Lysate )
Related Product Information for anti-RELB antibody
Description: Rabbit IgG polyclonal antibody for Transcription factor RelB(RELB) detection. Tested with WB in Human, Mouse, Rat.
Background: RELB(v-rel reticuloendotheliosis viral oncogene homolog B) is also known as IREL. The International Radiation Hybrid Mapping Consortium assigned the RELB gene to chromosome 19. By RT-PCR and immunocytochemical analyses, Clark et al.(1999) showed that RELB expression correlated with dendritic cell activation. NF-kappa-B-inducing kinase is required for osteoclastogenesis in response to pathologic stimuli. Vaira et al.(2008) found that overexpression of Relb, but not Rela, rescued differentiation of mouse Nik -/- osteoclast precursors, indicating that blockade of the alternative NF-kappa-B pathway, rather than the classical NF-kappa-B pathway, is responsible for the osteoclastogenic defect in the absence of Nik. Using Relb -/- mice, they showed that Relb itself was required for Rankl-induced osteoclastogenesis in vitro and for TNF-induced bone resorption in vivo. Both Relb -/- and Nik -/- mice were resistant to tumor-mediated osteolysis. Vaira et al.(2008) concluded that the alternative NF-kappa-B pathway, via RELB, plays an essential and unique role in RANKL signaling toward osteoclast development.
Background: RELB(v-rel reticuloendotheliosis viral oncogene homolog B) is also known as IREL. The International Radiation Hybrid Mapping Consortium assigned the RELB gene to chromosome 19. By RT-PCR and immunocytochemical analyses, Clark et al.(1999) showed that RELB expression correlated with dendritic cell activation. NF-kappa-B-inducing kinase is required for osteoclastogenesis in response to pathologic stimuli. Vaira et al.(2008) found that overexpression of Relb, but not Rela, rescued differentiation of mouse Nik -/- osteoclast precursors, indicating that blockade of the alternative NF-kappa-B pathway, rather than the classical NF-kappa-B pathway, is responsible for the osteoclastogenic defect in the absence of Nik. Using Relb -/- mice, they showed that Relb itself was required for Rankl-induced osteoclastogenesis in vitro and for TNF-induced bone resorption in vivo. Both Relb -/- and Nik -/- mice were resistant to tumor-mediated osteolysis. Vaira et al.(2008) concluded that the alternative NF-kappa-B pathway, via RELB, plays an essential and unique role in RANKL signaling toward osteoclast development.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
62,134 Da
NCBI Official Full Name
Transcription factor RelB
NCBI Official Synonym Full Names
v-rel avian reticuloendotheliosis viral oncogene homolog B
NCBI Official Symbol
RELB
NCBI Official Synonym Symbols
IREL; I-REL; REL-B
NCBI Protein Information
transcription factor RelB; v-rel reticuloendotheliosis viral oncogene homolog B, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)
UniProt Protein Name
Transcription factor RelB
Protein Family
UniProt Gene Name
RELB
UniProt Entry Name
RELB_HUMAN
Uniprot Description
RELB: a transcription factor of the nuclear factor-kappaB (NFkB) transcription complex. Stimulates promoter activity in the presence NFkB1/NFkB-p50. Plays role in regulation of apoptosis in hepatocellular carcinoma through activation of downstream target genes. Does not associate with DNA or RelA/NFkB-p65.
Protein type: Transcription factor
Chromosomal Location of Human Ortholog: 19q13.32
Cellular Component: nucleoplasm; cytoplasm; microtubule organizing center; cytosol
Molecular Function: protein binding; chromatin binding; transcription factor activity; transcription corepressor activity
Biological Process: transcription from RNA polymerase II promoter; antigen processing and presentation; I-kappaB kinase/NF-kappaB cascade; response to cytokine stimulus; T-helper 1 cell differentiation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; myeloid dendritic cell differentiation; negative regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; inflammatory response
Research Articles on RELB
Similar Products
Product Notes
The RELB relb (Catalog #AAA176199) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rel B antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Rel B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the RELB relb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rel B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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